Paenibacillus albicereus sp. nov. and Niallia alba sp. nov., isolated from digestive syrup

Two aerobic, Gram-stain variable, catalase-positive and oxidase-negative rods named strain UniB2(T) and UniB3(T), were isolated from digestive syrup containing fungal diastase (10 mg/ml), pepsin (2 mg/ml) and sugar base containing polyethylene glycol. Based on 16S rRNA gene sequence analysis, strain UniB2(T) has the highest sequence similarity with Paenibacillus humicus NBRC 102415 T (98.3%) and strain UniB3(T) showed the highest sequence similarity with Niallia circulans DSM 11(T) (98.9%). The DNA G+C content of UniB2(T) was 63.7 mol %. The dDDH and ANI values between the strain UniB2(T) and its phylogenetically close relative were < 38.3% and< 89.5%, respectively. The major fatty acids of the strain UniB2(T) were C-16:0 (13.9%), C-15:0 anteiso (39.7%), C-17:0 anteiso (15.5%). The DNA G+C content of UniB3(T) was 35.6 mol %. The dDDH and ANI values between the strain UniB3(T) and its close relatives were < 29.1% and 84.6%, respectively. The major fatty acids of strain UniB3(T) were C-16:0 (13.5%), C-15:0 anteiso (40.1%) and C-17:0 anteiso (16.0%). Major polar lipids for both strains were Diphosphatidylglycerol and phosphatidylethanolamine. Both strains showed unique carbon utilization and assimilation pattern that differentiated them from their phylogenetically related neighbours. These phenotypic, genotypic and chemotaxonomic characters indicated the strains UniB2(T) and UniB3(T) represent two novel species for which the names Paenibacillus albicereus sp. nov. (Type strain UniB2(T) =MCC 3997(T) =KCTC 43095(T) =JCM34513(T) ) and Niallia alba sp. nov. (Type strain UniB3(T) =MCC 3998(T) =KCTC 43235(T) =JCM 34492(T)) are proposed.

Automated summary

Two novel strains, UniB2(T) and UniB3(T), were isolated from digestive syrup and identified as new species of Paenibacillus and Niallia, respectively. These strains differed from their phylogenetically related neighbors in terms of fatty acid composition, DNA G+C content, and carbon utilization pattern.