期刊
AQUATIC TOXICOLOGY
卷 241, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.aquatox.2021.106005
关键词
Identification; Japanese flounder; mapk gene family; biotic and abiotic stresses
资金
- National Key Research and Development Program of China [2018YFD0900601]
This study identified 14 putative mapk genes in Japanese flounder, which were divided into three main subfamilies: ERK, JNK, and p38 MAPK. Evolutionary analysis revealed strong purifying selection on mapk genes, indicating high conservation in gene structure and protein motifs. Additionally, expression patterns under different stresses suggested potential diverse functions of mapk genes in Japanese flounder.
Mitogen-activated protein kinase (MAPK) are a series of serine/threonine protein kinases showing evolutionary conservation, which can be activated by many stimulus signals and then transfer them from cell membrane to nucleus. MAPKs regulate a variety of biological processes, such as apoptosis, hormone signaling and immune response. In this study, 14 putative mapk genes in Japanese flounder were identified, and their basic physical and chemical properties were characterized. Phylogenetic analysis showed that mapk genes were divided into three main subfamilies, including ERK, JNK and the p38 MAPK. Selection pressure analysis revealed they were evolutionarily-constrained and undergone strong purifying selection. Gene structure and conserved protein motif comparison suggested high levels of conservation in members of mapk gene family. The expression patterns were further investigated in each embryonic and larval development stages and different tissues. In addition, RNA-seq analyses after bacteria and temperature stresses suggested mapk genes had different expression patterns. Three mapk genes showed significant differences in response to E. tarda challenge and five were induced significantly after temperature stress, indicating their potential functions. This systematic analysis provided valuable information for further understanding of the regulation mechanism of mapk gene family under different stresses in Japanese flounder.
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