4.5 Article

Development of a cost-effective and portable aeration freezing system for germplasm cryopreservation for eastern oysters and other aquatic species

期刊

AQUACULTURE RESEARCH
卷 53, 期 2, 页码 453-466

出版社

WILEY
DOI: 10.1111/are.15587

关键词

cooling rate; cryopreservation; eastern oysters; portable cooling device

资金

  1. Gulf States Marine Fisheries Commission [ACQ-210-039-2019-USM]
  2. National Institute of Food and Agriculture, United States Department of Agriculture (Hatch project) [FLA-FOR-005385]
  3. National Sea Grant Aquaculture Initiative Award [NA18OAR4170344]

向作者/读者索取更多资源

The study aimed to develop a cost-effective and portable freezing system for germplasm cryopreservation of fish and shellfish. Utilizing an aeration system, different cooling rates were achieved and had an impact on post-thaw sperm motility.
The goal was to develop a cost-effective and portable freezing system for germplasm cryopreservation of fish and shellfish. The objectives were to: (1) identify components for construction of a portable freezing system by evaluating the affecting factors of cooling rates; (2) generate a range of cooling rates through adjusting different variables; (3) compare cooling profiles generated by the aeration freezing system and a commercial programmable freezer, and (4) verify the effectiveness of the aeration freezing system by cryopreserving sperm of eastern oysters Crassostrea virginica. An aeration freezing system was constructed using a Styrofoam box as cooling chamber and an innovative aeration unit to create nitrogen vapour. A wide range of cooling rates (1.5-32.1 degrees C/min) were achieved by combinations of sample position at 2, 5, or 10 cm above liquid nitrogen surface, the temperature of nitrogen vapour (-150 or -180 degrees C), and aeration time of liquid nitrogen (0, 1, 2 min, or continuity). Compared to a programmable freezer, the aeration freezing system generated cooling profiles with significantly higher temperature for ice nucleation initiation. No difference in post-thaw sperm motility of eastern oysters were found between samples cooling in the aeration freezing system or a programmable freezer. Cooling rates of 10 or 15 degrees C/min from 4 degrees C to -80 degrees C yielded the highest post-thaw sperm motility regardless of the cooling devices. The aeration freezing system developed in this study has the potential for high-throughput sample processing, and the design allows easy construction by multiple users.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.5
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据