期刊
AQUACULTURE
卷 546, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.aquaculture.2021.737337
关键词
Acute hepatopancreatic necrosis disease (AHPND); Pathogenic stress; Penaeus monodon; Serotonin; Gene expression
资金
- Ministry of Education [LS2019853]
- Government of the People's Republic of Bangladesh under the program GRANT OF ADVANCED RESEARCH IN EDUCATION
The study showed that the pathogens VP and WSSV significantly reduced hemocyte counts and increased glucose and serotonin levels in the hemolymph of black tiger shrimp. These pathogenic treatments altered the expression pattern of candidate genes and affected the physiological and biochemical parameters, resulting in negative impacts on growth and mortality of the experimental shrimps.
The black tiger shrimp (Penaeus monodon) represents a major crustacean aquaculture species globally. Outbreaks of diseases caused by two particular pathogens, Vibrio parahemolyticus (VP) and white spot syndrome virus (WSSV) severely hamper the production performance of this species. The present study investigated the effects of different doses of the two pathogens on physiological/biochemical parameters and differential gene expression in P. monodon. This study involved 10 different experimental groups including nine treatments (three doses of VP: T-1 - T-3, three doses of WSSV: T-4 - T-6 and three combined VP + WSSV doses: T-7 - T-9) and a control. VP and WSSV treatments significantly reduced hemocyte counts of shrimp hemolymph (P < 0.05). Different treatments showed 2-4 fold increase in hemolymph glucose and serotonin levels over the control shrimps. Pathogenic treatments significantly altered expression pattern of the selected set of candidate genes. The a-amylase (growth gene) showed reduced expression (1.5-3 fold lower) in treatments while immune response genes (Prophenoloxidase and Lysozyme) showed significantly higher expression levels (2-3 fold higher) in treatment groups compared to the control. Significantly higher O-2 consumption rates in the pathogen-treated shrimp, T-1 - T-9 (P < 0.05) indicate that these shrimp faced pathogenic stress. Shrimp in the control group showed 1.5-2 fold higher growth (increase in body weight) compared to treatment groups (T-1 - T-9). Results indicate that different doses of pathogenic exposure significantly altered the expression of candidate genes together with changes in physiological (O-2 consumption rates) and biochemical (hemocyte counts, glucose and serotonin levels of hemolymph) parameters that adversely affected growth and mortality of experimental shrimps.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据