期刊
AQUACULTURE
卷 547, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.aquaculture.2021.737456
关键词
A. schlegelii; Fatty acid restoration; Fish oil re-feeding strategy; Lipid metabolism
资金
- National Natural Science Foun-dation of China [31802303]
- National Key R & D Program of China [2018YFD0900400]
- Fundamental Research Funds for the Provincial Universities of Zhejiang [SJLY2021007]
- Scientific Research Foundation of Ningbo University [XYL20007]
- Industrial Chain Collaborative Inno-vation Project of the Demonstration Work on Innovative Development of the Marine Economy of the State Oceanic Administration [NBHY-2017-S2]
- Key Research Program of Zhejiang Province of China [2018C02037]
- Zhejiang Aquaculture Nutrition & Feed Technology Service Team [ZJANFTST2017-2]
- Open Fund of Zhejiang Provincial Top Key Discipline of Aquaculture in Ningbo University
- Ningbo University
The study indicated that a strategy of re-feeding fish oil following feeding black seabream with linseed oil for four weeks promoted growth, mitigated lipid accumulation in muscle, and effectively restored muscle n-3 LC-PUFA levels in A. schlegelii.
An eight-week feeding trial was conducted to evaluate the influence of an alternating linseed oil (LO) and fish oil (FO) feeding strategy in black seabream A. schlegelii (initial weight, 8.49 +/- 0.01 g). Two isonitrogenous and isolipidic feeds were formulated to contain FO or LO, and used to enable three feeding strategy treatments termed FO (feeding the FO diet for 8 weeks), LO (feeding the LO diet for 8 weeks) and FO re-feeding (feeding LO for 4 weeks then FO for 4 weeks). Fish in the FO re-feeding and FO groups had significantly higher final body weights (FBW), weight gains (WG), specific growth rates (SGR) but lower muscle lipid content than fish in the LO group. In muscle, the FO re-feeding strategy restored eicosapentaenoic and docosahexaenoic acid contents to 82.67% and 83.84%, respectively, of those in fish fed the FO treatment. In addition, the FO re-feeding strategy significantly down-regulated the expression levels of genes related to long-chain polyunsaturated fatty acid (LC-PUFA) biosynthesis and lipogenesis, including fatty acyl desaturase (fads2), elongase of very long-chain fatty acids 4a (elovl4a) and sterol regulatory element-binding protein-1 (srebp-1) compared to fish fed the LO treatment. Similarly, the hepatic Srebp-lc concentration in fish in the FO re-feeding group was markedly lower than in fish in the LO treatment group. Expression of the lipid catabolism gene adipose triglyceride lipase (atgl) was up-regulated in fish in the FO re-feeding group compared with fish in the LO group. Overall, the present study indicated that a strategy of re-feeding FO following feeding LO for four-week promoted growth, mitigated lipid accumulation in muscle, and effectively restored muscle n-3 LC-PUFA levels in A. schlegelii.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据