期刊
AQUACULTURE
卷 546, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.aquaculture.2021.737284
关键词
RNAi; DNA virus; Penaeus; miRNA; AMO
资金
- Mahidol University
- Royal Golden Jubilee Ph.D. Program [PHD/0316/2551]
- Thailand Science Research and Innovation [RSA6280096]
The study found that wsv-miR-9 plays an important role in early viral gene expression, leading to a delay in viral replication when its expression is inhibited. This highlights the potential for wsv-miR-9 as a target for controlling WSSV infection in shrimp.
Disease especially caused by White spot syndrome virus (WSSV) has a huge impact on the shrimp production industry. An understanding of shrimp-WSSV interaction could lead to an effective approach to control WSSV infection. MicroRNA (miRNA) is a short small RNA which functions in post-transcriptional gene regulation and is involved in the WSSV infection. Previously, WSSV-encoded miRNAs were identified from infected P. monodon. We investigated wsv-miR-9 which showed the highest expression in WSSV-infected gill tissue. The expression of wsv-miR-9 was up-regulated upon WSSV infection and reduced in moribund shrimp. Antisense inhibition using anti-miRNA oligonucleotides (AMO) resulted in a complete inhibition of wsv-miR-9 expression. An in vitro functional study demonstrated that mimic of wsv-miR-9 could function in translational suppression by decreasing the reporter protein activity when compared to the mutant target. In shrimp, loss of wsv-miR-9 by AMO9 resulted in a delay of viral replication after 6-12 h post WSSV challenge. A reduction of WSSV early genes, ie1 and DNA polymerase mRNA expressions was observed within 6 h. These results reveal that wsv-miR-9 is important in maintaining early viral gene expression.
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