Comparison of growth, survival and fertility of the southern and northern populations of Crassostrea ariakensis and their hybrids in southern China

Crassostrea ariakensis is widely distributed in the estuaries of China and has the advantages of wide salinity adaptation and strong resistance, but the artificial aquaculture of C. ariakensis is mainly concentrated in southern China. Long-term geographical isolation resulted in genetic variation between the northern and southern C. ariakensis populations. In this study, the reproductive isolation between two independent population of C. ariakensis was evaluated by hybridization. Results showed that the fertilization rate, cleavage rate and D Larval rate of interpopulation hybrids (NS-northern male x southern female and SN- southern male x northern female) were significantly lower than those of intrapopulation inbreed groups (SS and NN). Moreover, southern maternal larvae (SS and NS) have significant survival advantages. The growth trend between the two sites was very different in early stage (from 90th to 210th day), but it was consistent in late stage (from 210th to 480th day), with a significant decreasing trend of SS > NS > SN > NN. The survival rates of SN and NS was significantly higher than that of NN, indicating that the high temperature tolerance and stress resistance of hybrids were improved. The positive maternal effect advantages (MEAs) at most time points of both sites demonstrated that parental origin, especially the maternal origin, had a significant effect on the survival and growth of progeny. Hybrid advantages (HAs) for growth of both sites were opposite, and positive HAs of survival accounted for most of both sites, indicating that environmental conditions and survival could significantly affect the growth, and interpopulation hybrids did not necessarily always show positive HAs. Moreover, the maternal origin would affect the shell shape and shell hardness, resulted in NS similar to SS and SN similar to NN, respectively. Additionally, all interpopulation hybrids could produce mature gametes. These results confirmed that there was no serious reproductive isolation between the two populations. The southern maternal groups, especially SS, had better growth and survival advantages, could be used as good new strains for large-scale breeding in southern China, and also provided basic data for the commercial promotion of C. ariakensis in southern China.

Automated summary

The study evaluated the reproductive isolation between two populations of Crassostrea ariakensis by hybridization, finding that interpopulation hybrids showed lower fertilization, cleavage, and larval rates than intrapopulation inbreed groups. Southern maternal larvae had significant survival advantages and growth trends varied in early stage but were consistent in late stage. Interpopulation hybrids showed improved heat tolerance and stress resistance, with maternal origin having a significant effect on survival and growth of progeny.