期刊
AQUACULTURE
卷 547, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.aquaculture.2021.737473
关键词
Bacillus cereus; Soft-shelled turtle; Pathogenicity; Antibiotic susceptibility
资金
- National Natural Science Foundation of China [31802208, 31972646]
The soft-shelled turtle, an important aquacultural species in China, has been suffering from a bacterial infection caused by Bacillus cereus. This pathogen led to significant pathological changes in infected turtles, and the bacterium showed sensitivity to certain antibiotics, which could be used for prevention and treatment.
Soft-shelled turtle (Trionyx sinensis) is one of the most important aquacultural species in China. In recent years, an infectious disease frequently occurred in soft-shelled turtle, which exhibited similar symptoms, including craned necks, ulcerated pores on shells, hemorrhagic intestinal mucosa and heart, intumescent liver and spleen. In this study, a Gram-positive rod bacterial strain BC12 with vigorous hemolytic and lipolytic activity isolated from the sick soft-shelled turtles. Strain BC12 was identified as Bacillus cereus via 16S rRNA phylogenetic analysis and species-specific gene amplification. The juvenile soft-shelled turtles challenged with B. cereus BC12 exhibited similar symptoms as those natural infections, and the 50% lethal dose (LD50) is 3.89 x 10(5) colony-forming unit (CFU). Pure B. cereus re-isolated from the heart and liver of turtles infected with BC12. Noticeable pathological changes, including necrosis in the heart and liver and damage in the germinal center of the spleen, were observed in the B. cereus BC12 infected turtles for the first time. Antibiotics susceptibility assay showed that B. cereus BC12 is sensitive to the aminoglycosides, polypeptides, most of the cephalosporins and quinolones, which might be chosen as candidates to prevent and treat B. cereus infection.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据