期刊
AQUACULTURE
卷 548, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.aquaculture.2021.737644
关键词
Mytilus chilensis; Illumina Miseq; Gut microbiome; Aquaculture; 16SrDNA
资金
- Universidad Austral de Chile, Chile [DID 2016-07]
- Interdisciplinary Center for Aquaculture Research, INCAR, Chile [15110027]
The gut microbiome of Mytilus chilensis was analyzed using NGS, revealing differences in microbial communities between mussels from a farm and wild type individuals. The study showed a high diversity of gut microorganisms in Chilean mussels, with differences in dominant bacterial species among samples.
The gut microbiome of Mytilus chilensis was analyzed using a next-generation sequencing (NGS) approach using as target the V6-V8 16S rDNA gene regions. The resident gut bacterial communities were analyzed in individuals collected from two locations, both free-living in natural conditions (Wild Type group, WT) and those found in intensive commercial cultures at a Mussel Farm (MF group) off the Chilean coast. A total of 1.2 M of nonchimeric sequences were detected, belonging to 30 phyla, including Fusobacteria, Proteobacteria, Bacteroides, Tenericutes, and 404 genera taxa. The major fraction of the sequences detected corresponded to Vibrionaceae and Fusobacteriaceae families for MF and WT groups, respectively. The gut microbiome of M. chilensis showed a wide diversity of microorganisms; however, differences were observed depending on the origin of samples, where gut samples from the mussel farm showed a lower number of genera when compared to gut tissue samples collected from the wild type individuals. The dominant bacterial are Vibrio, Psychrilyobacter, Mycoplasma, and Psychromonas, that were present with different relative abundancies among the samples analyzed. This next generation sequencing technique, applied for the first time in Chilean mussel (M. chilensis), showed that the gut is a useful tissue for the study of bacterial communities in this bivalve mollusk. The results obtained in this work provided insights into the composition of the microbiota of M. chilensis, unravelling the great bacterial diversity and the effect of habitat on the bacterial community structure.
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