期刊
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
卷 106, 期 1, 页码 317-327出版社
SPRINGER
DOI: 10.1007/s00253-021-11673-5
关键词
Red yeasts; T-DNA insertional mutagenesis; Secondary metabolites; Lipids; Terpenoids
资金
- Slovak Ministry of Education, Science, Research and Sport [VEGA 1/0323/19]
- University of Sassari
- Sardinia Regional Government [C.U.P. J86C18000270002]
- COST [CA15136 EUROCAROTEN]
- PON AIM Program Azione I.2 Attrazione e Mobilita dei Ricercatori
Red yeasts, such as Rhodotorula, were used as biocatalysts for the production of commercial secondary metabolites, including lipids and carotenoids. Mutants with impaired carotenoid biosynthesis were isolated using T-DNA insertional mutagenesis, showing altered metabolic characteristics and increased production of specific compounds. This study demonstrates the potential for enhanced production of secondary metabolites through manipulation of carotenoid biosynthesis in red yeasts.
Red yeasts, mainly included in the genera Rhodotorula, Rhodosporidiobolus, and Sporobolomyces, are renowned biocatalysts for the production of a wide range of secondary metabolites of commercial interest, among which lipids, carotenoids, and other isoprenoids. The production of all these compounds is tightly interrelated as they share acetyl-CoA and the mevalonate pathway as common intermediates. Here, T-DNA insertional mutagenesis was applied to the wild type strain C2.5t1 of Rhodotorula mucilaginosa for the isolation of albino mutants with impaired carotenoids biosynthesis. The rationale behind this approach was that a blockage in carotenoid biosynthetic pathway could divert carbon flux toward the production of lipids and/or other molecules deriving from terpenoid precursors. One characterized albino mutant, namely, strain W4, carries a T-DNA insertion in the CAR1 gene coding for phytoene desaturase. When cultured in glycerol-containing medium, W4 strain showed significant decreases in cell density and fatty acids content in respect to the wild type strain. Conversely, it reached significantly higher productions of phytoene, CoQ(10), and sterols. These were supported by an increased expression of CAR2 gene that codes for phytoene synthase/lycopene cyclase. Thus, in accordance with the starting hypothesis, the impairment of carotenoids biosynthesis can be explored to pursue the biotechnological exploitation of red yeasts for enhanced production of secondary metabolites with several commercial applications.
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