期刊
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
卷 105, 期 24, 页码 9261-9272出版社
SPRINGER
DOI: 10.1007/s00253-021-11687-z
关键词
Adaptive; R; toruloides; Hydrolysate; Inhibitors; Lipids; Biodiesel
资金
- Brazilian National Council for Scientific and Technological Development (CNPq) [152033/2015-7]
- Sao Paulo Research Foundation (FAPESP) [2016/10636-8]
This study compared colony selection and heterologous adaptive laboratory enhancement (ALE) strategies for obtaining robust strains of the oleaginous yeast Rhodotorula toruloides, and found that the ALE strategy resulted in higher maximum growth rate, biomass production, and lipid content. Additionally, colony selection strategy helped shorten the lag phase. Moreover, kinetics studies showed that acetic acid acts as a repressor of xylose consumption during R. toruloides cultivation.
The oleaginous yeast Rhodotorula toruloides is a potential lipid producer for biodiesel production. However, this yeast shows growth inhibition due to harmful compounds when cultivated in hemicellulose hydrolysate. Here, we present a comparative analysis of colony selection and heterologous adaptive laboratory enhancement (ALE) strategies for obtaining robust strains. We implemented these ALE strategies for R. toruloides in a culture medium containing sugarcane hemicellulose hydrolysate. Our comparison study showed that the strain obtained with heterogeneous ALE strategy (Rth) reached a mu(max) of 55% higher than the parental strain. It also exhibited higher biomass production (6.51 g/l) and lipid content (60%). ALE with colony selection strategy (Rtc) had a fitness gain in terms of shortening of the lag phase (9 h) when compared to Rth and parental strain (11.67, 12.33 h, respectively). When cultivated in Eucalyptus urograndis hemicellulose hydrolysate, the Rth strain achieved a high lipid content, 64%. Kinetics studies showed a strong effect of acetic acid as a repressor of xylose consumption during R. toruloides cultivation.
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