期刊
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
卷 194, 期 6, 页码 2465-2480出版社
SPRINGER
DOI: 10.1007/s12010-022-03821-y
关键词
Mixed silage; Lactic acid bacteria; Cellulase; Peanut vines; Whole-plant corn; Anaerobic fermentation
资金
- Agroscientific Research in the Public Interest [201503134]
This study evaluated the effects of cellulase and Lactobacillus plantarum on the fermentation quality and bacterial community of whole-plant corn and peanut vine mixed silage. The results showed that the simultaneous addition of cellulase and Lactobacillus plantarum improved the fermentation quality of the mixed silage.
Significant anaerobic fermentation occurs in silage through the action of anaerobic bacteria. The objective of this study was to evaluate the effects of cellulase and Lactobacillus plantarum on the fermentation quality and bacterial community of whole-plant corn and peanut vine mixed silage. Mixed silage was tested with no addition (CK), addition of Lactobacillus plantarum (LP), addition of cellulase (CE), and the simultaneous addition of Lactobacillus plantarum and cellulase (LPCE). LPCE samples exhibited decreased pH; decreased content of acetic acid, propionic acid, and butyric acid; and increased content of lactic acid. LP and LPCE had better effects on chemical composition than CK and CE, especially in decreasing acid detergent fiber and neutral detergent fiber content. High-throughput sequencing identified Lactobacillus, Klebsiella, Serratia, and Weissella as the main microorganisms. LP and CE increased the abundance of Acetobacter, and LPCE decreased the abundance of Acetobacter. All additives decreased the abundance of Weissella, Leuconostoc, and Lactococcus, and increased the abundance of Pantoea. Overall, simultaneous addition of cellulase and Lactobacillus plantarum helped to improve the quality of mixed silage of whole-plant corn and peanut vines.
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