期刊
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
卷 87, 期 23, 页码 -出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.01855-21
关键词
Escherichia coli; ethanol; YghA; furfural; lignocellulose; tolerance
资金
- Indian Council of Medical Research
- Department of Biotechnology, Government of India, via Bioenergy Centre [BT/PR/Centre/03/2011-Phase II]
Overexpression of the yghA gene allows Escherichia coli to exhibit tolerance against furfural and increase ethanol production efficiency. YghA shows high activity against furfural when utilizing NADH as a cofactor.
Furfural is a common furan inhibitor formed due to dehydration of pentose sugars, like xylose, and acts as an inhibitor of microbial metabolism. Overexpression of NADH-specific FucO and deletion of NADPH-specific YqhD had been a successful strategy in the past in conferring tolerance against furfural in Escherichia coli, which highlights the importance of oxidoreductases in conferring tolerance against furfural. In a screen consisting of various oxidoreductases, dehydrogenases, and reductases, we identified the yghA gene as an overexpression target to confer tolerance against furfural. YghA preferably used NADH as a cofactor and had an apparent K-m value of 0.03 mM against furfural. In the presence of 1 g liter(-1) furfural and 10% xylose (wt/vol), yghA overexpression in an ethanologenic E. coli strain SSK42 resulted in an ethanol efficiency of similar to 97%, with a 53-fold increase in ethanol titers compared to the control. YghA also exhibited activity against the less toxic inhibitor 5-hydroxymethyl furfural, which is formed due to dehydration of hexose sugars, and thus is a formidable target for overexpression in ethanologenic strain for fermentation of sugars in biomass hydrolysate. IMPORTANCE Lignocellulosic biomass represents an inexhaustible source of carbon for second-generation biofuels. Thermo-acidic pretreatment of biomass is performed to loosen the lignocellulosic fibers and make the carbon bioavailable for microbial metabolism. The pretreatment process also results in the formation of inhibitors that inhibit microbial metabolism and increase production costs. Furfural is a potent furan inhibitor that increases the toxicity of other inhibitors present in the hydrolysate. Thus, it is desirable to engineer furfural tolerance in E. coli for efficient fermentation of hydrolysate sugars.
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