期刊
ANNALS OF SURGICAL ONCOLOGY
卷 29, 期 5, 页码 2914-2925出版社
SPRINGER
DOI: 10.1245/s10434-021-11157-w
关键词
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资金
- ASCO Equipment for the endowment for Excellence in Women's Health
- TTUHSC
This experiment evaluates the role of tumor-infiltrating lymphocytes (TILs) in distant tumors after cryoablation of breast cancer, and finds that cryoablation can induce a tumor-specific TIL response, preventing cancer recurrence and metastasis.
Background Morphological evaluation of tumor-infiltrating lymphocytes (TILs) in breast cancer is gaining momentum as an immunological biomarker. This experiment evaluates the role of TILs in distant tumors as a measure of abscopal effect from cryoablation of breast cancer. Methods BALB/c mice underwent bilateral orthotopic transplant with 4T1-12B (triple-negative) cells. At 2 weeks, left tumors were treated by either resection (standard of care group) or cryoablation (intervention group) followed by resection of the distant right tumors 1 week posttreatment. TIL scores were calculated from hematoxylin and eosin-stained sections and phenotyped for cytotoxic T-lymphocyte (CTL) markers by immunofluorescence. Primarily resected tumors served as baseline (T-baseline), whereas resected distant right-sided served as the readout for abscopal effect (Abs(Res) or Abs(Cryo)). Mice were monitored for tumor recurrence and metastasis. Results The Abs(Cryo) had a significant mean (SD) increase in stromal (2.8 [1.1]%; p = 0.015) and invasive margin TILs (50 [12]%; p = 0.02) compared with T-Baseline (1.0 [0]% and 31 [4.9]%, respectively). CTL phenotyping revealed a significant increase in mean (SD) CD8(+) T cells (15.7 [12.1]; p = 0.02) and granzyme B (4.8 [3.6]; p = 0.048) for the Abs(Cryo) compared with T-Baseline (5.2 [4.7] and 2.4 [0.9], respectively). Posttreatment, the cryoablation group had no recurrence or metastasis, whereas the resected group showed local recurrence and lung metastasis in 40% of the mice. Postprocedure increase in TIL score of distant tumors was associated with decrease in tumor relapse (p = 0.02). Conclusions Cryoablation induced a robust tumor-specific TIL response compared with resection, suggesting an abscopal effect leading to the prevention of cancer recurrence and metastasis.
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