4.4 Review

Advances in sperm cryopreservation in farm animals: Cattle, horse, pig and sheep

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Article Biology

Effect of Extender, Storage Time and Temperature on Kinetic Parameters (CASA) on Bull Semen Samples

Aitor Fernandez-Novo et al.

Summary: The study showed that CASA kinetic parameters remain stable up to 4-6 hours post-ejaculation, suggesting prompt assessment of seminal quality. BIOXcell(R) preserves sperm motility reliably at 5 degrees C, while INRA96(R) maintains sperm motility at room temperature.

BIOLOGY-BASEL (2021)

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Effect of autologous platelet-rich plasma on human sperm quality during cryopreservation

Bo Yan et al.

Summary: The study found that adding 5% autologous PRP can improve sperm motility, viability, and membrane integrity after freezing-thawing, suggesting a partial protective effect of autologous PRP on cryopreservation of human spermatozoa.

CRYOBIOLOGY (2021)

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Use of antioxidants to augment semen efficiency during liquid storage and cryopreservation in livestock animals: A review

Mohsen Ghaleb Al-Mutary

Summary: Despite the widespread use of frozen or refrigerated mammalian spermatozoa, their quality remains low due to sensitivity to injuries during preservation and thawing. Antioxidants have been employed in livestock spermatozoa preservation to maintain sperm quality and counteract oxidative stress.

JOURNAL OF KING SAUD UNIVERSITY SCIENCE (2021)

Article Agriculture, Dairy & Animal Science

Influence of seminal plasma during different stages of bovine sperm cryopreservation

Gabriela Bertaiolli Zoca et al.

Summary: This study found that the presence of seminal plasma contributes to maintaining acrosomal integrity and reducing F-actin cytoskeleton remodelling in bovine sperm. Cooling stage induced the highest F-actin remodelling, while freezing/thawing stage caused the most damage to sperm membranes and mitochondrial potential.

REPRODUCTION IN DOMESTIC ANIMALS (2021)

Article Veterinary Sciences

Oxidative and nitrosative stress in frozen-thawed pig spermatozoa. I: Protective effect of melatonin and butylhydroxytoluene on sperm function

Felipe Pezo et al.

Summary: The addition of BHT and MEL to the cryopreservation medium reduces oxidative and nitrosative stress markers in frozen-thawed spermatozoa, which benefits the integrity of cell membranes.

RESEARCH IN VETERINARY SCIENCE (2021)

Article Cell Biology

Species-Specific Differences in Sperm Chromatin Decondensation Between Eutherian Mammals Underlie Distinct Lysis Requirements

Jordi Ribas-Maynou et al.

Summary: This study aimed to analyze the resilience of sperm DNA to decondensation in various eutherian mammals, and found that longer incubations in lysis solution including proteinase K led to higher DNA decondensation in porcine and bovine sperm. In species with both P1 and P2 protamines, the incubation time had no impact on DNA decondensation.

FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY (2021)

Article Reproductive Biology

Sperm-bound antisperm antibodies are associated with poor cryosurvival of stallion spermatozoa

M. S. Ferrer et al.

Summary: The presence of sperm-bound antisperm antibodies (ASAs) is associated with poor cryosurvival of stallion sperm, leading to lower post-thaw sperm motility, velocity, and higher percentage of apoptotic sperm. Stallions with ASAs show higher levels of IgG- and IgA-bound sperm and are more likely to have poor sperm cryosurvival. Evaluating stallions for the presence of ASAs before freezing may provide an indicator of their prognosis for cryosurvival.

THERIOGENOLOGY (2021)

Article Agriculture, Dairy & Animal Science

Effects of Apigenin and Astragalus Polysaccharide on the Cryopreservation of Bull Semen

Hongtao Wang et al.

Summary: The study investigated the effects of supplementing apigenin and astragalus polysaccharide on bull semen cryopreservation. Results showed that adding 0.2 mmol/L AP or 0.5 mg/mL APS alone improved the quality of frozen sperm, while a combination of the two further enhanced the protective effects on post-thaw semen quality.

ANIMALS (2021)

Article Agriculture, Dairy & Animal Science

Post-Thaw Sperm Quality and Functionality in the Autochthonous Pig Breed Gochu Asturcelta

Jose Nestor Caamano et al.

Summary: Genetic resource banks aim to preserve genetic material of endangered individuals or valuable breeds. This study focused on frozen-thawed sperm of Gochu Asturcelta pigs, finding season had a significant impact on sperm quality, with spring and summer samples showing higher quality. The post-thawing sperm quality was comparable to commercial breeds.

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In vitro assessment of egg yolk-, soya bean lecithin- and liposome-based extenders for cryopreservation of dairy bull semen

Sara Miguel-Jimenez et al.

ANIMAL REPRODUCTION SCIENCE (2020)

Review Biochemistry & Molecular Biology

Sperm Cryodamage in Ruminants: Understanding the Molecular Changes Induced by the Cryopreservation Process to Optimize Sperm Quality

Patricia Peris-Frau et al.

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A novel approach for human sperm cryopreservation with AFPIII

Saeed Zandiyeh et al.

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Cryopreservation Induces Alterations of miRNA and mRNA Fragment Profiles of Bull Sperm

Aishao Shangguan et al.

FRONTIERS IN GENETICS (2020)

Article Agriculture, Dairy & Animal Science

Effect of astaxanthin in extenders on sperm quality and functional variables of frozen-thawed boar semen

A. Basioura et al.

ANIMAL REPRODUCTION SCIENCE (2020)

Article Agriculture, Dairy & Animal Science

Optimization of cryopreservation protocols for cooled-transported stallion semen

M. S. Ferrer et al.

ANIMAL REPRODUCTION SCIENCE (2020)

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Cellular and Functional Physiopathology of Bull Sperm With Altered Sperm Freezability

Mustafa Hitit et al.

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Effect of Moringa oleifera seed extract on antioxidant activity and sperm characteristics in cryopreserved ram semen

Jose Maria Carrera-Chavez et al.

JOURNAL OF APPLIED ANIMAL RESEARCH (2020)

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Impact of post-thaw supplementation of semen extender with antioxidants on the quality and function variables of stallion spermatozoa

Favian Treulen et al.

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Exploration of miRNA and mRNA Profiles in Fresh and Frozen-Thawed Boar Sperm by Transcriptome and Small RNA Sequencing

Ding-Hui Dai et al.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2019)

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The proteome of frozen-thawed pig spermatozoa is dependent on the ejaculate fraction source

Cristina Perez-Patino et al.

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Cryopreservation Differentially Alters the Proteome of Epididymal and Ejaculated Pig Spermatozoa

Cristina Perez-Patino et al.

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The Use of Antifreeze Proteins in the Cryopreservation of Gametes and Embryos

Vanesa Robles et al.

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GSTM3, but not IZUMO1, is a cryotolerance marker of boar sperm

Marc Llavanera et al.

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Biochemical basis and metabolic interplay of redox regulation

Lixiao Zhang et al.

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Extenders modify the seminal plasma ability to minimize freeze-thaw damage on ram sperm

Rafael Ramirez-Vasquez et al.

REPRODUCTION IN DOMESTIC ANIMALS (2019)

Article Agriculture, Dairy & Animal Science

Aquaglyceroporins but not orthodox aquaporins are involved in the cryotolerance of pig spermatozoa

Ariadna Delgado-Bermudez et al.

JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY (2019)

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Specific Activity of Superoxide Dismutase in Stallion Seminal Plasma Is Related to Sperm Cryotolerance

Marion Papas et al.

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Redox Regulation and Oxidative Stress: The Particular Case of the Stallion Spermatozoa

Fernando J. Pena et al.

ANTIOXIDANTS (2019)

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Cryopreservation of horse semen with a liposome and trehalose added extender

Ariadna Z. Medina-Leon et al.

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Oxidative stress and sperm function: A systematic review on evaluation and management

Sulagna Dutta et al.

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Effect of reduced glutathione supplementation on cryopreservation induced sperm cryoinjuries in Murrah bull semen

Chetna Gangwar et al.

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The Effect of Four Different Commercial Semen Extenders on the Motility of Stallion Epididymal Sperm

Stefanie Neuhauser et al.

JOURNAL OF EQUINE VETERINARY SCIENCE (2018)

Article Biochemical Research Methods

Cryopreservation and egg yolk medium alter the proteome of ram spermatozoa

T. Pini et al.

JOURNAL OF PROTEOMICS (2018)

Review Developmental Biology

CASA-Mot in mammals: an update

J. L. Yaniz et al.

REPRODUCTION FERTILITY AND DEVELOPMENT (2018)

Review Developmental Biology

CASA-Mot technology: how results are affected by the frame rate and counting chamber

Daznia Bompart et al.

REPRODUCTION FERTILITY AND DEVELOPMENT (2018)

Article Developmental Biology

Effect of counting chamber depth on the accuracy of lensless microscopy for the assessment of boar sperm motility

Carles Soler et al.

REPRODUCTION FERTILITY AND DEVELOPMENT (2018)

Editorial Material Developmental Biology

Computer-assisted sperm analysis and reproductive science; a gift for understanding gamete biology from multidisciplinary perspectives

William V. Holt et al.

REPRODUCTION FERTILITY AND DEVELOPMENT (2018)

Article Agriculture, Dairy & Animal Science

Effects of two freezing methods and two cryopreservation media on post-thaw quality of stallion spermatozoa

S. Macedo et al.

REPRODUCTION IN DOMESTIC ANIMALS (2018)

Article Veterinary Sciences

Using Resveratrol and Epigallocatechin-3-Gallate to Improve Cryopreservation of Stallion Spermatozoa With Low Quality

Houshang Nouri et al.

JOURNAL OF EQUINE VETERINARY SCIENCE (2018)

Review Obstetrics & Gynecology

Sperm cryopreservation: A review on current molecular cryobiology and advanced approaches

Maryam Hezavehei et al.

REPRODUCTIVE BIOMEDICINE ONLINE (2018)

Article Veterinary Sciences

Evaluation of bull spermatozoa during and after cryopreservation: Structural and ultrastructural insights

Wael A. Khalil et al.

INTERNATIONAL JOURNAL OF VETERINARY SCIENCE AND MEDICINE (2018)

Article Developmental Biology

Effects of reduced glutathione on acrosin activity in frozen-thawed boar spermatozoa

Efren Estrada et al.

REPRODUCTION FERTILITY AND DEVELOPMENT (2017)

Article Obstetrics & Gynecology

New permeable cryoprotectant-free vitrification method for native human sperm

J. Aizpurua et al.

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Article Developmental Biology

Aquaglyceroporins 3 and 7 in bull spermatozoa: identification, localisation and their relationship with sperm cryotolerance

Noelia Prieto-Martinez et al.

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Article Agriculture, Dairy & Animal Science

Aquaporins in the male reproductive tract and sperm: Functional implications and cryobiology

M. Yeste et al.

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Article Reproductive Biology

Cryopreservation of bull semen is associated with carbonylation of sperm proteins

Agnieszka Mostek et al.

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Article Agriculture, Dairy & Animal Science

Impact of holding and equilibration time on post-thaw quality of shipped boar semen

J. Schaefer et al.

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Sperm subpopulations in ejaculated sperm and spermatozoa recovered from ovine epididymides up to 48 h after death

Tacia Gomes Bergstein-Galan et al.

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Seminal plasma proteins modify the distribution of sperm subpopulations in cryopreserved semen of rams with lesser fertility

Alba Ledesma et al.

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Storage temperature and sucrose concentrations affect ram sperm quality after vitrification

A. Arando et al.

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Cholesterol-loaded cyclodextrin improves ram sperm cryoresistance in skim milk-extender

Vianney M. Salmon et al.

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Cytosine methylation of sperm DNA in horse semen after cryopreservation

Christine Aurich et al.

THERIOGENOLOGY (2016)

Article Agriculture, Dairy & Animal Science

Functional aspects of seminal plasma and sperm proteins and their potential as molecular markers of fertility

A. A. Moura et al.

ANIMAL REPRODUCTION (2016)

Article Agriculture, Dairy & Animal Science

Cryopreservation of stallion spermatozoa using different cryoprotectants and combinations of cryoprotectants

Zhuangyuan Wu et al.

ANIMAL REPRODUCTION SCIENCE (2015)

Article Biochemical Research Methods

The identification of proteomic markers of sperm freezing resilience in ram seminal plasma

J. P. Rickard et al.

JOURNAL OF PROTEOMICS (2015)

Review Agriculture, Dairy & Animal Science

Recent Advances in Boar Sperm Cryopreservation: State of the Art and Current Perspectives

M. Yeste

REPRODUCTION IN DOMESTIC ANIMALS (2015)

Article Biochemistry & Molecular Biology

Paternal RNA contributions in the Caenorhabditis elegans zygote

Marlon Stoeckius et al.

EMBO JOURNAL (2014)

Article Agriculture, Dairy & Animal Science

Function of ram spermatozoa frozen in diluents supplemented with casein and vegetable oils

I. Del Valle et al.

ANIMAL REPRODUCTION SCIENCE (2013)

Article Veterinary Sciences

Addition of Glutathione to an Extender for Frozen Equine Semen

Rodrigo Arruda de Oliveira et al.

JOURNAL OF EQUINE VETERINARY SCIENCE (2013)

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Is Sperm Freezability Related to the Post-Thaw Lipid Peroxidation and the Formation of Reactive Oxygen Species in Boars?

J. Gomez-Fernandez et al.

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Article Agriculture, Dairy & Animal Science

Seminal plasma proteins increase the post-thaw sperm viability of Sanmartinero bull's semen

Fabian Rueda A et al.

REVISTA MVZ CORDOBA (2013)

Article Agriculture, Dairy & Animal Science

Soybean lecithin-based extender as an alternative for goat sperm cryopreservation

Andrea Helena Vidal et al.

SMALL RUMINANT RESEARCH (2013)

Article Agriculture, Dairy & Animal Science

In vitro evaluation of ram sperm frozen with glycerol, ethylene glycol or acetamide

E. C. B. Silva et al.

ANIMAL REPRODUCTION SCIENCE (2012)

Review Biology

Freezing injury: The special case of the sperm cell

G. John Morris et al.

CRYOBIOLOGY (2012)

Review Agriculture, Dairy & Animal Science

Seminal Plasma Proteins as Modulators of the Sperm Function and Their Application in Sperm Biotechnologies

I. Caballero et al.

REPRODUCTION IN DOMESTIC ANIMALS (2012)

Article Reproductive Biology

Improvement of boar sperm cryosurvival by using single-layer colloid centrifugation prior freezing

M. J. Martinez-Alborcia et al.

THERIOGENOLOGY (2012)

Article Reproductive Biology

Effects of reactive oxygen species on sperm function

H. D. Guthrie et al.

THERIOGENOLOGY (2012)

Article Immunology

Seminal Plasma Proteins: What Role Do They Play?

Heriberto Rodriguez-Martinez et al.

AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY (2011)

Article Agriculture, Dairy & Animal Science

Replacing egg yolk with soybean lecithin in the cryopreservation of stallion semen

Frederico Ozanam Papa et al.

ANIMAL REPRODUCTION SCIENCE (2011)

Article Biochemistry & Molecular Biology

Relationships Between the Dynamics of Iatrogenic DNA Damage and Genomic Design in Mammalian Spermatozoa From Eleven Species

Jaime Gosalvez et al.

MOLECULAR REPRODUCTION AND DEVELOPMENT (2011)

Article Agriculture, Dairy & Animal Science

In Vitro and In Vivo Evaluation of Ram Sperm Frozen in Tris Egg-yolk and Supplemented with Superoxide Dismutase and Reduced Glutathione

S. V. Silva et al.

REPRODUCTION IN DOMESTIC ANIMALS (2011)

Article Reproductive Biology

Liposomes for cryopreservation of bovine sperm

T. Roepke et al.

THERIOGENOLOGY (2011)

Article Reproductive Biology

Egg yolk plasma can replace egg yolk in stallion freezing extenders

E. Pillet et al.

THERIOGENOLOGY (2011)

Review Reproductive Biology

Statistical Series: Opportunities and challenges of sperm motility subpopulation analysis

Felipe Martinez-Pastor et al.

THERIOGENOLOGY (2011)

Article Andrology

Carbohydrate-and heparin-binding proteins in mammalian fertilization

E. Töpfer-Petersen et al.

ANDROLOGIA (2010)

Article Agriculture, Dairy & Animal Science

Alterations of sperm DNA integrity during cryo preservation procedure and in vitro incubation of bull semen

K. E. Waterhouse et al.

ANIMAL REPRODUCTION SCIENCE (2010)

Article Reproductive Biology

Osmotic Stress Induces Oxidative Cell Damage to Rhesus Macaque Spermatozoa

Megan J. McCarthy et al.

BIOLOGY OF REPRODUCTION (2010)

Article Developmental Biology

Seminal plasma proteins inhibit in vitro- and cooling-induced capacitation in boar spermatozoa

Melissa L. Vadnais et al.

REPRODUCTION FERTILITY AND DEVELOPMENT (2010)

Review Obstetrics & Gynecology

Implication of apoptosis in sperm cryoinjury

Tamer M. Said et al.

REPRODUCTIVE BIOMEDICINE ONLINE (2010)

Article Agriculture, Dairy & Animal Science

Cryoprotectant effect of trehalose and low-density lipoprotein in extenders for frozen ram semen

R. A. Tonieto et al.

SMALL RUMINANT RESEARCH (2010)

Article Reproductive Biology

The HSP90AA1 sperm content and the prediction of the boar ejaculate freezability

I. Casas et al.

THERIOGENOLOGY (2010)

Article Agriculture, Dairy & Animal Science

Evaluation of L-glutamine for cryopreservation of boar spermatozoa

E. de Mercado et al.

ANIMAL REPRODUCTION SCIENCE (2009)

Article Agriculture, Dairy & Animal Science

Identification of Sperm Subpopulations in Stallion Ejaculates: Changes after Cryopreservation and Comparison with Traditional Statistics

C. Ortega-Ferrusola et al.

REPRODUCTION IN DOMESTIC ANIMALS (2009)

Article Obstetrics & Gynecology

Influence of freeze-thawing on hyaluronic acid binding of human spermatozoa

Martine Nijs et al.

REPRODUCTIVE BIOMEDICINE ONLINE (2009)

Article Agriculture, Dairy & Animal Science

Cryobiological determinants of frozen semen quality, with special reference to stallion

H. Sieme et al.

ANIMAL REPRODUCTION SCIENCE (2008)

Article Developmental Biology

Sperm-derived histones contribute to zygotic chromatin in humans

Godfried W. van der Heijden et al.

BMC DEVELOPMENTAL BIOLOGY (2008)

Article Agriculture, Dairy & Animal Science

Seminal plasma proteins and sperm resistance to stress

T. Muino-Blanco et al.

REPRODUCTION IN DOMESTIC ANIMALS (2008)

Article Reproductive Biology

Genetics and genomics to improve fertility in high producing dairy cows

R. F. Veerkamp et al.

THERIOGENOLOGY (2007)

Article Agriculture, Dairy & Animal Science

Fertility of ewes inseminated intrauterinally with frozen semen using extender containing bovine serum albumin

Yutaka Fukui et al.

JOURNAL OF REPRODUCTION AND DEVELOPMENT (2007)

Article Reproductive Biology

Seasonal variations in antioxidant enzyme activity in ram seminal plasma

E. Marti et al.

THERIOGENOLOGY (2007)

Article Agriculture, Dairy & Animal Science

Effect of low density lipoprotein on DNA integrity of freezing-thawing boar sperm by neutral comet assay

Zhong-Liang Jiang et al.

ANIMAL REPRODUCTION SCIENCE (2007)

Review Ecology

Semen cryopreservation and the conservation of endangered species

Joerns Fickel et al.

EUROPEAN JOURNAL OF WILDLIFE RESEARCH (2007)

Article Reproductive Biology

Impact of antifreeze proteins and antifreeze glycoproteins on bovine sperm during freeze-thaw

N. S. Prathalingam et al.

THERIOGENOLOGY (2006)

Article Agriculture, Dairy & Animal Science

Factors influencing boar sperm cryosuirvival

J. Roca et al.

JOURNAL OF ANIMAL SCIENCE (2006)

Article Agriculture, Dairy & Animal Science

Effects of bovine serum albumin and trehalose in semen diluents for improvement of frozen-thawed ram spermatozoa

Taiki Matsuoka et al.

JOURNAL OF REPRODUCTION AND DEVELOPMENT (2006)

Article Agriculture, Dairy & Animal Science

The role of stallion seminal proteins in fertilisation

E Töpfer-Petersen et al.

ANIMAL REPRODUCTION SCIENCE (2005)

Article Agriculture, Dairy & Animal Science

Amides as cryoprotectants for freezing stallion semen: A review

MA Alvarenga et al.

ANIMAL REPRODUCTION SCIENCE (2005)

Review Dentistry, Oral Surgery & Medicine

Platelet-rich plasma: Evidence to support its use

RE Marx

JOURNAL OF ORAL AND MAXILLOFACIAL SURGERY (2004)

Article Agriculture, Dairy & Animal Science

Comparative effects of autologous and homologous seminal plasma on the viability of largely extended boar spermatozoa

I Caballero et al.

REPRODUCTION IN DOMESTIC ANIMALS (2004)

Article Reproductive Biology

The role of osmotic resistance on equine spermatozoal function

AC Pommer et al.

THERIOGENOLOGY (2002)

Article Biology

Structure and function of antifreeze proteins

PL Davies et al.

PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES (2002)

Review Neurosciences

Aquaporin water channels - from atomic structure to clinical medicine

P Agre et al.

JOURNAL OF PHYSIOLOGY-LONDON (2002)

Article Obstetrics & Gynecology

The effects of cryopreservation on sperm morphology, motility and mitochondrial function

M O'Connell et al.

HUMAN REPRODUCTION (2002)

Article Reproductive Biology

Semen plasma proteins prevent cold-shock membrane damage to ram spermatozoa

R Pérez-Pé et al.

THERIOGENOLOGY (2001)

Review Agriculture, Dairy & Animal Science

Basic aspects of frozen storage of semen

WV Holt

ANIMAL REPRODUCTION SCIENCE (2000)