期刊
ANIMAL BIOTECHNOLOGY
卷 -, 期 -, 页码 -出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/10495398.2021.1976198
关键词
Animal welfare; bioinformatics; electrical stunning; halal; proteomics; mass spectrometry; 2-dimensional gel electrophoresis
资金
- Indian National Science Academy (INSA), Government of India [LF121]
- Department of Science and Technology (DST), Government of India [LF121]
The study showed that slaughtering of sheep without stunning resulted in changes in protein abundance related to various physiological processes. These protein alterations have the potential to serve as indicators for assessing animal welfare.
The commercial production of halal and kosher meat and controversy surrounding the slaughter without stunning is rapidly growing across the globe. Huge global market for halal and kosher meat warrants conciliation of religious practices and animal welfare for the betterment of meat industry. In the present study, we investigated changes in muscle proteome of sheep (Ovis aries) subjected to either electrical stunning and slaughtering or slaughter without any stunning (halal). The 2DE gel analysis detected approximately 377 protein spots in which 243 (119 up regulated and 124 down regulated) protein spots were significantly (p <= 0.05) differentially expressed with a fold change ratio >= 1.5/<= 1.5. The in-gel digestion and MALDI-TOF/TOF MS analysis of statistically significant protein spots revealed 35 differentially abundant proteins out of which 26 were up-regulated and 9 were down-regulated. The study demonstrated that slaughtering of sheep without stunning resulted in changes in the abundance of proteins involved in catalytic, structural, and stress related processes. This understanding of protein alterations in sheep slaughtered with and without stunning have the potential to act as possible signature for animal welfare index.
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