期刊
ANIMAL BIOTECHNOLOGY
卷 34, 期 4, 页码 921-934出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/10495398.2021.2003373
关键词
Dibutyryl-cAMP; preadipocytes; proliferation; differentiation; adipogenesis; fat deposition
This study investigates the mechanism of dbcAMP on porcine fat deposition. The results suggest that dbcAMP regulates gene expression related to adipocyte differentiation and fat metabolism through the cAMP-PKA pathway, leading to reduced fat deposition.
This study investigated potential mechanism of dibutyryl-cAMP (db-cAMP) on porcine fat deposition. (1) Exp.1, 72 finishing pigs were allotted to 3 treatments (0, 10 or 20 mg/kg dbcAMP) with 6 replicates. dbcAMP increased the hormone sensitive lipase (HSL) activity and expression of beta-adrenergic receptor (beta-AR) and growth hormone receptor (GHR), but decreased expression of peroxisome proliferator-activated receptor gamma 2 (PPAR-gamma 2) and adipocyte fatty acid binding protein (A-FABP) in back fat. dbcAMP upregulated expression of beta-AR, GHR, PPAR-gamma 2 and A-FABP, but decreased insulin receptor (INSR) expression in abdominal fat. Dietary dbcAMP increased HSL activity and expression of G protein-coupled receptor (GPCR), cAMP-response element-binding protein (CREB) and insulin-like growth factor-1 (IGF-1), but decreased fatty acid synthase (FAS) and lipoprotein lipase (LPL) activities, and expression of INSR, cAMP-response element-binding protein (C/EBP-alpha) and A-FABP in perirenal fat. (2) Exp. 2, dbcAMP suppressed the proliferation and differentiation of porcine preadipocytes in a time- and dose-dependent manner, which might be associated with increased activities of cAMP and protein kinase A (PKA), and expression of GPCR, beta-AR, GHR and CREB via inhibiting C/EBP-alpha and PPAR-gamma 2 expression. Collectively, dbcAMP treatment may reduce fat deposition by regulating gene expression related to adipocyte differentiation and fat metabolism partially via cAMP-PKA pathway.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据