期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 61, 期 13, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.202112232
关键词
Cholesterol; Inward-Rectifier K+ Channels; Membrane Proteins; NMR Structure; Solid-State NMR
资金
- NIH [P41GM136463, P41GM111135]
- Wisconsin Alumni Research Foundation
- University of Wisconsin-Madison
- National Institutes of Health (Maximizing Investigators' Research Award) [R35, 1R35GM124979]
Cholesterol oligomers are found in various membrane protein crystal structures, but their biological function is not well understood. This study presents the structural and functional details of a cholesterol dimer that stabilizes the inactivated state of an inward-rectifier potassium channel. The findings provide insights into the role of cholesterol oligomers in regulating channel conductance.
Cholesterol oligomers reside in multiple membrane protein X-ray crystal structures. Yet, there is no direct link between these oligomers and a biological function. Here we present the structural and functional details of a cholesterol dimer that stabilizes the inactivated state of an inward-rectifier potassium channel KirBac1.1. K+ efflux assays confirm that high cholesterol concentration reduces K+ conductance. We then determine the structure of the cholesterol-KirBac1.1 complex using Xplor-NIH simulated annealing calculations driven by solid-state NMR distance measurements. These calculations identified an alpha-alpha cholesterol dimer docked to a cleft formed by adjacent subunits of the homotetrameric protein. We compare these results to coarse grain molecular dynamics simulations. This is one of the first examples of a cholesterol oligomer performing a distinct biological function and structural characterization of a conserved promiscuous lipid binding region.
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