Cathespin B-Initiated Cypate Nanoparticle Formation for Tumor Photoacoustic Imaging

Cathepsin B (CTSB) is a lysosomal protease that is overexpressed in the early stage of many cancer types. Precise evaluation of CTSB expression in vivo may provide a promising method for the early diagnosis of cancers. By virtue of the high-resolution PA imaging modality, a smart photoacoustic (PA) probe Cypate-CBT, which can self-assemble to cypate-containing nanoparticles in response to abundant GSH and CTSB inside tumor cells, was developed for the sensitive and specific detection of CTSB activity. Compared with unmodified Cypate, our probe Cypate-CBT exhibited a 4.9-fold or 4.7-fold PA signal enhancement in CTSB-overexpressing MDA-MB-231 cancer cells or tumors, respectively, revealing intracellular accumulation of the probe after CTSB-initiated self-assembly. We expect Cypate-CBT to be employed as an effective PA imaging agent for clinical diagnosis of cancer at early stages.

Automated summary

CTSB is overexpressed in many types of cancer in the early stage, and the smart photoacoustic probe Cypate-CBT can achieve sensitive and specific detection of CTSB activity, showing intracellular accumulation after self-assembly initiated by CTSB. It is expected to be an effective PA imaging agent for early cancer diagnosis.