4.8 Article

Light-Harvesting Fluorescent Spherical Nucleic Acids Self-Assembled from a DNA-Grafted Conjugated Polymer for Amplified Detection of Nucleic Acids

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出版社

WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.202115812

关键词

Light-Harvesting Antenna; MicroRNA Detection; Spherical Nucleic Acid; pi-Conjugated Polymer

资金

  1. National Natural Science Foundation of China [51973089]
  2. Shenzhen Science and Technology Innovation Commission [KQTD20170810111314625]

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In this study, a new type of spherical nucleic acids (SNAs) was developed by using fluorescent pi-conjugated polymers (FCPs) as light-harvesting antennas to enhance signal transduction in nucleic acid detection. The hydrophobicity of the graft copolymer was adjusted to control the size and light-harvesting capability of the FCP-SNAs. The optimized FCP-SNA showed a 37-fold signal amplification effect and a limit of detection as low as 1.7 pM in microRNA detection. This method was successfully applied for amplified in situ nucleic acid detection and imaging at the single-cell level.
The ultralow concentration of nucleic acids in complex biological samples requires fluorescence probes with high specificity and sensitivity. Herein, a new kind of spherical nucleic acids (SNAs) is developed by using fluorescent pi-conjugated polymers (FCPs) as a light-harvesting antenna to enhance the signal transduction of nucleic acid detection. Specifically, amphiphilic DNA-grafted FCPs are synthesized and self-assemble into FCP-SNA structures. Tuning the hydrophobicity of the graft copolymer can adjust the size and light-harvesting capability of the FCP-SNAs. We observe that more efficient signal amplification occurs in larger FCP-SNAs, as more chromophores are involved, and the energy transfer can go beyond the Forster radius. Accordingly, the optimized FCP-SNA shows an antenna effect of up to 37-fold signal amplification and the limit of detection down to 1.7 pM in microRNA detection. Consequently, the FCP-SNA is applied to amplified in situ nucleic acid detecting and imaging at the single-cell level.

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