期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 61, 期 13, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.202114632
关键词
Analytical Methods; Antimicrobial Peptides; Cell-Free Protein Synthesis; Droplet Microfluidics; Membranes
资金
- European Research Council [681587]
- Swiss National Science Foundation (NCCR Molecular Systems Engineering) [51NF40-182895]
- Eidgenossische Technische Hochschule Zurich
- Swiss National Science Foundation (SNF) [51NF40-182895] Funding Source: Swiss National Science Foundation (SNF)
- European Research Council (ERC) [681587] Funding Source: European Research Council (ERC)
Researchers have developed a novel microfluidic system that enables the simultaneous production and screening of membrane-specific antimicrobial peptides. This system provides a valuable tool for the discovery and development of membrane-active antimicrobials.
The global surge in bacterial resistance against traditional antibiotics triggered intensive research for novel compounds, with antimicrobial peptides (AMPs) identified as a promising candidate. Automated methods to systematically generate and screen AMPs according to their membrane preference, however, are still lacking. We introduce a novel microfluidic system for the simultaneous cell-free production and screening of AMPs for their membrane specificity. On our device, AMPs are cell-free produced within water-in-oil-in-water double emulsion droplets, generated at high frequency. Within each droplet, the peptides can interact with different classes of co-encapsulated liposomes, generating a membrane-specific fluorescent signal. The double emulsions can be incubated and observed in a hydrodynamic trapping array or analyzed via flow cytometry. Our approach provides a valuable tool for the discovery and development of membrane-active antimicrobials.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据