期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 60, 期 52, 页码 26947-26953出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.202108714
关键词
density-based assays; magnetic immunoassays; sink; float magnetic immunoassays; sink; float tests
资金
- University of Massachusetts Lowell through start-up funds
- Tripathy Memorial Graduate Fellowship Award (University of Massachusetts Lowell)
- Graduate Research Grant Award (Graduate Student Association, University of Massachusetts Lowell)
This study presents a new type of sink/float magnetic immunoassays for the rapid detection of biomarkers in untreated biological samples, which are simple to operate and may meet regulatory authorities' criteria for field use.
Analytical tests/devices that are used outside laboratory settings are required to have a very simple analytical protocol to get clearance by regulatory authorities. This study describes sink/float magnetic immunoassays, a new type of rapid, mix-and-observe, instrument-free tests for the detection of biomarkers in untreated biological samples that are very simple and might meet the simple-to-use criterion of authorities to be used in the field. These tests can tell whether an analyte is above or below a predetermined level within 25-45 minutes based on the sinking or floating of a mm-sized sphere on the surface of which an immunoassay that uses reporter antibodies conjugated to superparamagnetic nanoparticles is performed. This manuscript describes the theory and proof-of-concept applications of sink/float magnetic immunoassays for the detection of C-Reactive Protein, anti-Treponema pallidum antibodies and E. coli bacteria.
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