期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 61, 期 10, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.202113477
关键词
Chemical Reaction Network; Dissipative Self-Assembly; DNA Nanoscience; DNAzyme; Transient Catalysis
资金
- European Research Council [677960]
- Projekt DEAL
- European Research Council (ERC) [677960] Funding Source: European Research Council (ERC)
The development of synthetic non-equilibrium systems has opened doors for man-made life-like materials. Through ATP-driven dynamic covalent DNA assembly, we have successfully achieved ATP-fueled transient organization and ATP-fueled DNAzymes for substrate cleavage.
The development of synthetic non-equilibrium systems opens doors for man-made life-like materials. Yet, creating distinct transient functions from artificial fuel-driven structures remains a challenge. Building on our ATP-driven dynamic covalent DNA assembly in an enzymatic reaction network of concurrent ATP-powered ligation and restriction, we introduce ATP-fueled transient organization of functional subunits for various functions. The programmability of the ligation/restriction site allows to precisely organize multiple sticky-end-encoded oligo segments into double-stranded (ds) DNA complexes. We demonstrate principles of ATP-driven organization into sequence-defined oligomers by sensing barcode-embedded targets with different defects. Furthermore, ATP-fueled DNAzymes for substrate cleavage are achieved by transiently ligating two DNAzyme subunits into a dsDNA complex, rendering ATP-fueled transient catalytic function.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据