期刊
ANDROLOGIA
卷 54, 期 2, 页码 -出版社
WILEY
DOI: 10.1111/and.14320
关键词
autophagy; ferroptosis; necroptosis; pyroptosis; scrotal hyperthermia
类别
资金
- Urology and Nephrology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran [27068]
This study aimed to evaluate the signaling pathway involved in cell death after testicular hyperthermia. The results showed that heat stress disrupts spermatogenesis by activating several non-apoptotic signaling pathways in testicular tissue.
Cell death is a biologically uncontrollable and regulated process associated with human diseases which usually occur in response to oxidative stress that activates signalling pathways in multiple forms and can therefore contribute to human diseases. Thus, the current study aims to evaluate the signalling pathway involved in cell death after testicular hyperthermia. For this purpose, 32 mice were equally divided into four groups; I: Control; II, III and IV, Scrotal hyperthermia in which the testes are exposed to water at 43 degrees C for 20 min every other day, respectively, 15, 10 and 5 times. Then, animals were euthanized and testicular tissue samples were isolated to evaluate protein expression as well as germ cell gene marker expression by Western blot and real-time PCR tests. Our data showed that the protein expression of Caspase-1, Beclin1, Atg7, Mlkl and Acsl4 together with the expression of Caspase-1, Beclin1, Atg7, Mlkl and Acsl4 genes was significantly up-regulated in scrotal hyperthermia-induced mice. In conclusion, the present study showed that heat stress disrupts spermatogenesis by activating several non-apoptotic signalling pathways in testicular tissue.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据