期刊
ANALYTICAL LETTERS
卷 55, 期 11, 页码 1701-1718出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/00032719.2021.2023170
关键词
Agricultural by-products; indirect competitive enzyme-linked immunosorbent assay (ic-ELISA); lateral-flow immunochromatographic (LFIC) strip; methyl parathion; triazophos
资金
- Beijing University of Chemical Technology-China-Japan Friendship Hospital Biomedical Transformation Engineering Research Center Joint Project [RZ2020-02]
- AECC Beijing Institution of Aeronautical Materials
- National Key Research and Development Program of China [2016YFF0203703]
The ic-ELISA and LFIC strip were developed for the determination of methyl parathion and triazophos in agricultural by-products, providing simple and practical methods for rapid detection of organophosphorus pesticides. The LFIC strip was found to be suitable for on-site testing and rapid screening.
Residues of pesticides in agricultural by-products may cause harm to human health. An indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and a competitive form of lateral-flow immunochromatographic (LFIC) strip were developed for the determination of methyl parathion and triazophos in agricultural by-products. In the optimized system for ic-ELISA, the limits of detection (LODs) for methyl parathion and triazophos were 1.39 and 1.94 ng/mL. The linear ranges for methyl parathion and triazophos were from 7.90 to 200 ng/mL and 4.47 to 170.19 ng/mL. The recoveries from agricultural by-products ranged from 84.16 to 106.96% and the coefficients of variation were from 0.77 to 6.45%. In the optimized LFIC system, good linearity was obtained for methyl parathion and triazophos between 5 and 200 ng/mL. The LODs of the CG-LFIC strip for methyl parathion and triazophos were 2.17 and 4.21 ng/mL. The coefficients of variation of the test strip were from 0.73 to 8.34%. Pears, apples, cucumbers, and lettuces spiked with methyl parathion and triazophos were employed to characterize matrix effects using the LFIC strip. The ic-ELISA and LFIC strip were simple and practical for the rapid determination of organophosphorus pesticides in agricultural by-products. Furthermore, the LFIC strip was suitable for on-site testing and rapid screening.
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