期刊
ANALYTICAL CHEMISTRY
卷 93, 期 41, 页码 13783-13790出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.1c02284
关键词
-
资金
- NSFC [22074039, 21305041, 21705045]
- Training Program for Huxiang Youth Science and Technology Innovation Talents [2018RS3062]
- Hunan Provincial Natural Science Foundation [2019JJ50385]
- Hunan Provincial Education Department Project [19C1153]
- Training Program for Excellent Young Innovators of Changsha [KQ2009043]
This study presents a new strategy for sensitive PEC immunoassay by modifying the surface charge of photoactive materials. By utilizing CaCO3 nanoparticles as labels to regulate the surface charge of CdS@HMP QDs, sensitive detection of CEA is achieved with a linear detection range of 0.005-50 ng mL(-1) and a detection limit of 1 pg mL(-1).
The development of efficient signaling strategies is highly important for photoelectrochemical (PEC) immunoassay. We report here a new and efficient strategy for sensitive PEC immunoassay by tailoring the electrostatic interaction between the photoactive material and the electron donor. The photoelectric conversion of hexametaphosphate (HMP)-capped CdS quantum dots (QDs) in Na2SO3 solution is significantly boosted after Ca2+ incubation. The negative surface charges on CdS@HMP QDs decrease because of the complexation reaction between HMP and Ca2+, and the electrostatic repulsion between CdS@HMP QDs and electron donor (SO32-) becomes weak accordingly, leading to an improved electron-hole separation efficiency. Inspired by the PEC response of CdS@HMP QDs to Ca2+, a novel signal-on PEC immunoassay platform is established by employing CaCO3 nanoparticles as labels. By regulating the surface charge of CdS@HMP QDs with in situ-generated Ca2+ from CaCO3 labels, sensitive detection of the carcinoembryonic antigen (CEA) is achieved. The linear detection range is 0.005-50 ng mL(-1) and the detection limit is 1 pg mL(-1) for CEA detection. Our work not only provides a facile route to tailor the photoelectric conversion but also lays the foundation for sensitive PEC immunoassay by simply regulating the surface charge of photoactive materials.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据