Smartphone-Based Janus Micromotors Strategy for Motion-Based Detection of Glutathione

Herein, we describe a Janus micromotor smartphone platform for the motion-based detection of glutathione. The system compromises a universal three-dimensional (3D)-printed platform to hold a commercial smartphone, which is equipped with an external magnification optical lens (20-400x) directly attached to the camera, an adjustable sample holder to accommodate a glass slide, and a light-emitting diode (LED) source. The presence of glutathione in peroxide-rich sample media results in the decrease in the speed of 20 mu m graphene-wrapped/PtNPs Janus micromotors due to poisoning of the catalytic layer by a thiol bond formation. The speed can be correlated with the concentration of glutathione, achieving a limit of detection of 0.90 mu M, with percent recoveries and excellent selectivity under the presence of interfering amino acids and proteins. Naked-eye visualization of the speed decrease allows for the design of a test strip for fast glutathione detection (30 s), avoiding previous amplification strategies or sample preparation steps. The concept can be extended to other micromotor approaches relying on fluorescence or colorimetric detection for future multiplexed schemes.

Automated summary

The Janus micromotor smartphone platform described in this study enables motion-based detection of glutathione, with a limit of detection of 0.90 μM. The speed decrease of micromotors in the presence of glutathione allows for naked-eye visualization and fast detection without the need for amplification strategies or sample preparation steps, showing excellent sensitivity and selectivity in the presence of interfering substances.