期刊
ANALYTICAL CHEMISTRY
卷 94, 期 9, 页码 3881-3887出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.1c04847
关键词
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资金
- National Natural Science Foundation of China [62105184, 22101158, 22175033]
- Natural Science Foundation of Shandong Province, China [ZR2021QB043, ZR2021MB088]
- Special Fund of Taishan Scholars Project of Shandong Province, China [tsqn201909012]
- Natural Science Foundation of Jiangsu Province, China [BK20210107]
- Guangdong Basic and Applied Basic Research Foundation [2020A1515110578]
- Program of Qilu Young Scholars of Shandong University
In this study, we developed a novel fluorescent probe ANI for wash-free visualization of lipid droplets and fatty liver disease characteristics. ANI showed enhanced fluorescence in low-polar environments, allowing for selective lighting up of lipid droplets with high contrast. The probe successfully demonstrated the excessive accumulation of lipid droplets in a fatty liver disease model mouse.
The prominent pathological feature of fatty liver disease lesions is excessive fat accumulation in lipid droplets in hepatocytes. Thus, developing fluorescent lipid droplet-specific probes with high permeability and a high imaging contrast provides a robust tool for diagnosing fatty liver diseases. Herein, we rationally developed a novel donor-acceptor lipophilic fluorescent probe ANI with high photostability for wash-free visualization of lipid droplets and fatty liver disease characteristics. ANI showed a typical twisted intramolecular charge transfer effect with very faint fluorescence in high-polar solvents, but dramatically boosted emissions in low-polar environments. The solvatochromic probe can selectively light up lipid droplets with a high contrast in a wash-free manner. Further use of ANI to reveal the excessive accumulation of lipid droplets with a significantly large size in the liver tissues from the fatty liver disease model mice was successfully demonstrated. The remarkable imaging performances rendered ANI an alternative tool for accurately evaluating fatty liver disease in intraoperative diagnosis.
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