期刊
ANALYTICAL CHEMISTRY
卷 93, 期 51, 页码 17110-17118出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.1c04608
关键词
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资金
- Natural Science Foundation of Zhejiang Province [LQ19B050004]
- Basic Public Welfare Research Project of Zhejiang Province [LGG19B050001]
- Open Research Fund of the Key Laboratory of the Ministry of Education for Advanced Catalysis Materials (Zhejiang Normal University)
Efforts have been made to search for highly efficient electrochemiluminescence (ECL) emitters for clinical diagnosis and food safety applications. Triazinyl-based hydrogen bond organic frameworks (Tr-HOFs) were synthesized and used as a novel ECL luminophore to construct a label-free biosensor for kanamycin detection, showing promising results in biomedical analysis and food detection.
Nowadays, continuous efforts have been devoted to searching highly efficient electrochemiluminescence (ECL) emitters for applications in clinical diagnosis and food safety. In this work, triazinyl-based hydrogen bond organic frameworks (Tr-HOFs) were synthesized by N center dot center dot center dot H hydrogen bond self-assembly aggregation, where 6,6'-(1,4-phenylene)bis(1,3,5-triazine-2,4-diamine) (phenyDAT) was prepared via the cyclization reaction and behaved as a novel ligand. Impressively, the resulting Tr-HOFs showed strong ECL responses with highly enhanced ECL efficiency (21.3%) relative to the Ru(bpy)(3)(2+) standard, while phenyDAT hardly showed any ECL emission in aqueous phase. The Tr-HOFs innovatively worked as a new ECL luminophore to construct a label-free biosensor for assay of kanamycin (Kana). Specifically, the ECL response greatly weakened upon assembly of captured DNA with ferrocene (cDNA-Fc) onto the Tr-HOFs-modified electrode, while the ECL signals were adversely recovered by releasing linked DNA (L-DNA) from double-stranded DNA (dsDNA, hybridization of aptamer DNA (aptDNA) with L-DNA) due to the specific recognition of Kana with the aptDNA combined by the linkage of L-DNA and cDNA-Fc on the electrode. The as-built sensor showed a broadened linear range (1 nM-10 mu M) and a limit of detection (LOD) down to 0.28 nM, which also displayed satisfactory results in the analysis of Kana in the milk and diluted human serum samples. This work offers a novel pathway to design an ECL emitter with organic molecules, holding great promise in biomedical analysis and food detection.
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