期刊
ANALYTICAL CHEMISTRY
卷 93, 期 51, 页码 16987-16994出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.1c02131
关键词
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资金
- National Institutes of Health (NIH) [5R01NS112176-02]
- National Research Foundation of Korea (NRF) - Korean government (MSIT) [NRF 2021R1A2B5B02002437]
N-MCSWV is a novel voltammetric technique that allows quantitative measurements of extracellular levels of serotonin and other substances in vivo. When used with PEDOT:Nafion-coated carbon fiber microelectrodes, it can continuously monitor tonic serotonin concentrations in the brain of rats.
Here, we present the development of a novel voltammetric technique, N-shaped multiple cyclic square wave voltammetry (N-MCSWV) and its application in vivo. It allows quantitative measurements of tonic extracellular levels of serotonin in vivo with mitigated fouling effects. N-MCSWV enriches the electrochemical information by generating high dimensional voltammograms, which enables high sensitivity and selectivity against 5-hydroindoleacetic acid (5-HIAA), dopamine, 3,4-dihydroxyphenylacetic acid (DOPAC), histamine, ascorbic acid, norepinephrine, adenosine, and pH. Using N-MCSWV, in combination with PEDOT:Nafion-coated carbon fiber microelectrodes, a tonic serotonin concentration of 52 +/- 5.8 nM (n = 20 rats, +/- SEM) was determined in the substantia nigra pars reticulata of urethane-anesthetized rats. Pharmacological challenges with dopaminergic, noradrenergic, and serotonergic synaptic reuptake inhibitors supported the ability of N-MCSWV to selectively detect tonic serotonin levels in vivo. Overall, N-MCSWV is a novel voltammetric technique for analytical quantification of serotonin. It offers continuous monitoring of changes in tonic serotonin concentrations in the brain to further our understanding of the role of serotonin in normal behaviors and psychiatric disorders.
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