Rapid Cyanobacteria Species Identification with High Sensitivity Using Native Mass Spectrometry

Cyanobacteria have evolved over billions of years to adapt and survive in diverse climates. Environmentally, this presents a huge challenge because cyanobacteria can now rapidly form algae blooms that are detrimental to aquatic life. In addition, many cyanobacteria produce toxins, making them hazardous to animals and humans that they encounter. Rapid identification of cyanobacteria is essential to monitor and prevent toxic algae blooms. Here, we show for the first time how native mass spectrometry can quickly and precisely identify cyanobacteria from diverse aquatic environments. By monitoring phycobiliproteins, abundant protein complexes within cyanobacteria, simple, easy-to-understand mass spectral fingerprints were created that were unique to each species. Moreover, our method is 10-fold more sensitive than the current MALDI-TOF mass spectrometric methods, meaning that cyanobacteria can be monitored using this technology prior to bloom formation. Together, the data show great promise for the simultaneous detection and identification of co-existing cyanobacteria in situ.

Automated summary

This study demonstrates the rapid and precise identification of cyanobacteria from diverse aquatic environments using native mass spectrometry, creating unique mass spectral fingerprints by monitoring phycobiliproteins. The method is 10-fold more sensitive than current mass spectrometric methods, allowing for monitoring of cyanobacteria before bloom formation with great potential for simultaneous detection and identification of co-existing cyanobacteria in situ.