期刊
ANALYTICAL CHEMISTRY
卷 94, 期 3, 页码 1769-1777出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.1c04468
关键词
-
资金
- National Natural Science Foundation of China [81872835, 21621003, 21563010]
- Health Major Project [BWS17J028, AWS16J018]
Researchers developed a strategy to construct a kinetically orthogonal probe labeled KOP, which can measure the concentrations of nitroreductase and H2S and accurately indicate the invasiveness of tumors in a cellular model with complex H2S generation pathway.
The concentrations of nitroreductase and H2S have been widely used to predict the invasiveness of tumors. However, the above two substrates always interfere with the measurement of each other as both substrates react with the typical nitroaromatic probe with the same process. Moreover, the above interferences may lead to the misjudgment of the tumor invasiveness. We used a strategy combining kinetical distinguishing and signal amplification to construct a kinetically orthogonal probe labeled KOP. The above strategy expanded the gap between the reactivity of KOP to H2S and nitroreductase with an acceptable reactivity and could determine the concentration of coexisting nitroreductase and H2S on a kinetic curve with a breakpoint. KOP could also indicate the correct invasiveness tendency in the cellular model with a complex H2S generation pathway, while the traditional kinetically nonorthogonal probe could not indicate invasiveness correctly.
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