期刊
ANALYTICAL CHEMISTRY
卷 94, 期 4, 页码 2313-2320出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.1c05047
关键词
-
资金
- National Natural Science Foundation of China [21627809, 21827812]
- Special Foundation for Taishan Scholar Professorship of Shandong Province [TS201712052]
- Innovation Team Project of Colleges and Universities in Jinan [2019GXRC027]
This study proposed a strategy to improve the ECL performance of Au nanoclusters by introducing L-Cys, which limits the intramolecular motion and nonradiative relaxation of the excited state. Triethylamine was used as a coreactant to further enhance the ECL performance.
Screening new electrochemiluminescence (ECL) emitters for the design of sensitive detection strategies with even long emission wavelength is intensively anticipated in ECL evolution. Herein, a promising modification strategy for improving the ECL performance of Au nanoclusters (AuNCs) as a watersoluble luminophore was proposed. Upon the introduction of Lcysteine (L-Cys) onto the surface of glutathione (GSH)-stabilized AuNCs (GSH-AuNCs), the dual-thiol bond between L-Cys and GSH was formed to limit the intramolecular motion and nonradiative relaxation of the excited state from the capping agents, which resulted in the enhancement of monochromatic ECL emission of GSH-AuNCs with a red-shifted wavelength. By utilizing triethylamine as a coreactant, the ECL of L-Cys/GSHAuNCs was about 1.5-fold stronger than that of GSH-AuNCs, and the emission wavelength red-shifted from 660 to 780 nm at a relatively low potential, which could decrease the interference in bioassay and the photochemical damage in nondestructive detection. As a proof of application, a sandwich-type immunosensing method for CYFRA 21-1 was proposed with L-Cys/GSHAuNCs as the signal tag, which displayed a wide linear ranging from 0.2 fg/mL to 2 ng/mL and a limit of detection down to 0.067 fg/mL at 3S/N. This work provides a wonderful strategy for promoting the performance of ECL emitters.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据