期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 414, 期 3, 页码 1389-1402出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-021-03764-z
关键词
Distyrylbenzene derivative; Aggregation-induced emission; Electrochemistry; Electrochemiluminescence; Ratiometric immunosensor; CYFRA21-1
资金
- National Natural Science Foundation of China [21705084]
- Natural Science Foundation of Shandong Province of China [ZR2017BB074, ZR2018PB015]
- National Training Program of Innovation and Entrepreneurship for Undergraduates [S202010431027]
- Qilu University of Technology of Training Program of Innovation and Entrepreneurship for Undergraduates [xj201910431125]
- Innovation Team of Jinan City [2018GXRC004]
- Special Funds for Taishan Scholars Project
A novel ratiometric sandwich immunosensor for CYFRA21-1 detection was fabricated based on TPAPCN and 3D-GN/PtNPs/Th, achieving rapid and reliable detection through the ratio between ECL and electrochemical signals. The prepared sensor exhibited good linearity in the range of 50 fg/mL to 1 ng/mL with a detection limit as low as 16 fg/mL, showing significant application in clinical monitoring and biomolecules detection.
Aggregation-induced electrochemiluminescence reagent, a distyrylbenzene derivative with donor-acceptor conjugated nanosheet structure, namely TPAPCN, was used as a trace label and modified on the electrode through the formation of classical sandwich complex of antibody-antigen-antibody in this work. In aggregate state, TPAPCN with twisted structure was limited in nanometer space through intermolecular pi-pi stacking interactions, which not only restricts the intramolecular motions but also combines a large number of singlet excitons to greatly trigger electrochemiluminescence (ECL). The ECL signal of this system enhanced with more captured cytokeratin 19 fragment 21-1 (CYFRA21-1) on the modified electrode. Three-dimensional graphene/platinum nanoparticles with large specific surface, and excellent electroconductivity and biocompatibility were prepared and acted as excellent carriers for thionine handling (3D-GN/PtNPs/Th), which was employed for improving the loading of antibodies and generating internal electrochemical signal. Consequently, a novel ratiometric sandwich immunosensor for CYFRA21-1 detection was fabricated based on TPAPCN and 3D-GN/PtNPs/Th, that is, a rapid and reliable detection was achieved through the ratio between ECL and electrochemical signals. The prepared sensor performed good linearity in the range of 50 fg/mL to 1 ng/mL with a detection limit as low as 16 fg/mL. Moreover, the detection results revealed well in the analysis of human serum samples, demonstrating a significant application for clinical monitoring and biomolecules detection.
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