期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 413, 期 30, 页码 7451-7462出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-021-03703-y
关键词
Electrochemical aptasensor; Iron metal-organic framework (MIL-88); Graphene oxide; Zinc sulfide; Insulin detection; Methylene blue
An electrochemical aptasensor based on a single-stranded aptamer-Au@Fe-MIL-88 complex was developed for the sensitive and selective determination of insulin. The sensor demonstrated good sensitivity and reproducibility for detecting insulin in serum samples.
This work introduces an electrochemical aptasensor based on a single-stranded aptamer-Au@Fe-MIL-88 complex for sensitive and selective determination of insulin using differential pulls voltammetry. Au@Fe-MIL-88 with a large surface area was synthesized and employed as a suitable substrate for immobilization of the aptamer (APT-Au@Fe-MIL-88). Methylene blue (MB), as an electrochemical probe, was intercalated into the aptamer. Graphene oxide (GO) and zinc sulfide (ZnS) were placed on the Au electrode to amplify the MB current. Also, ZnS improves the immobilization of APT-Au@Fe-MIL-88 into the aptasensor through the strong interaction of Au-S. In the presence of the insulin, MB is released from the aptamer due to DNA conformational change, and as a result, the peak intensity of the intercalated MB was decreased. Under optimal conditions, the change in the current of MB was proportional to the insulin concentration in the range of 5.0 x 10(-16)-5.0 x 10(-11) mol L-1, with a superior ultra-low detection limit of 1.3 x 10(-16) mol L-1. It was observed that the aptasensor is suitable for determining insulin in serum samples with good sensitivity and reproducibility and with recoveries ranging from 96.4 to 102.0%. The relative standard deviations (RSD) were lower than 3.8% (n = 3). Graphic abstract
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