期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 414, 期 18, 页码 5071-5085出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-021-03751-4
关键词
Surface plasmon resonance; Silicon photonics; Antibody immobilization; Biochemical cross-linking; Antifouling coating; Lipid membrane
资金
- Spanish Ministry of Science and Innovation [PGC-2018-099870]
- European Regional Development Fund (ERDF)
- CERCA program/Generalitat de Catalunya
- Severo Ochoa Centers of Excellence program - AEI [SEV-2017-0706]
- Spanish Research Agency (AEI)
Label-free biosensors based on optical transducers offer potential alternatives for rapid and highly sensitive clinical diagnostics, but face challenges in standardizing sensor biofunctionalization protocols. Innovative strategies and research directions are crucial for enhancing the applicability and reliability of optical biosensors in clinics and biomedicine.
Label-free biosensors, and especially those based on optical transducers like plasmonic or silicon photonic systems, have positioned themselves as potential alternatives for rapid and highly sensitive clinical diagnostics, on-site environmental monitoring, and for quality control in foods or other industrial applications, among others. However, most of the biosensor technology has not yet been transferred and implemented in commercial products. Among the several causes behind that, a major challenge is the lack of standardized protocols for sensor biofunctionalization. In this review, we summarize the most common methodologies for sensor surface chemical modification and bioreceptor immobilization, discussing their advantages and limitations in terms of analytical sensitivity and selectivity, reproducibility, and versatility. Special focus is placed on the suggestions of innovative strategies towards antifouling and biomimetic functional coatings to boost the applicability and reliability of optical biosensors in clinics and biomedicine. Finally, a brief overview of research directions in the area of device integration, automation, and multiplexing will give a glimpse of the future perspectives for label-free optical biosensors.
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