A novel fluorescent platform of DNA-stabilized silver nanoclusters based on exonuclease III amplification-assisted detection of Salmonella Typhimurium

A novel fluorescent platform of DNA-stabilized silver nanoclusters (DNA-AgNCs) has been developed based on exonuclease III (Exo III) amplification-assisted for simple and sensitive detection of Salmonella Typhimurium (S. Typhimurium). The platform was designed by using magnetic beads, aptamer, its complementary DNA, hairpin probe (HP), Exo III, AgNO3, and NaBH4. The functionalized HP contained a cytosine-rich oligonucleotide loop (C-rich loop), which served as an effective template for the chemical reduction of Ag+ with NaBH4 to synthesize DNA-AgNCs. In the presence of S. Typhimurium, the C-rich loop was converted into an open form of ssDNA by the recycle digestion of Exo III, leading to a corresponding decrease in fluorescence intensity. Based on the fluorescence changes of the formed DNAAgNCs, the sensitive detection of S. Typhimurium was achieved. Under the optimal conditions, a wide linear relationship was observed in the concentration of S. Typhimurium ranging from 4.6 x 10(2) to 4.6 x 10(7) cfu mL(-1) with the limit of detection (LOD) being 82 cfu mL(-1). The method showed good selectivity for detecting S. Typhimurium. In addition, the platform could be used for the detection of S. Typhimurium in milk samples. The LOD reached 6.6 x 10(2) cfu mL(-1) with a good linear range, indicating that the method had excellent practicability in complex food samples. (c) 2021 Elsevier B.V. All rights reserved.

Automated summary

A novel fluorescent platform based on DNA-stabilized silver nanoclusters has been developed for the simple and sensitive detection of Salmonella Typhimurium using Exo III amplification. The platform utilizes a C-rich loop in the hairpin probe for the synthesis of DNA-AgNCs, with fluorescence changes enabling the detection of S. Typhimurium.