期刊
ANALYTICA CHIMICA ACTA
卷 1187, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.aca.2021.339138
关键词
Click chemistry; Functional polymers; Co-immobilization; Protein microarray; Peptide microarray; Extracellular vesicles
资金
- European Union [766466]
- Regione Lombardia, project READY (Regional Network for developing diagnostic methods in rapid response to emerging epidemics and bio-emergencies) [229472, 1157625]
A new polymeric coating was introduced to co-immobilize proteins and oriented peptides on the same substrate, facilitating the phenotype characterization of extracellular vesicles. Through NMR characterization and post polymerization modification, the strategy could reduce the number of analysis, save time, reagents, and costs.
The analytical performance of the microarray technique in screening the affinity and reactivity of molecules towards a specific target, is highly affected by the coupling chemistry adopted to bind probes to the surface. However, the surface functionality limits the biomolecules that can be attached to the surface to a single type of molecule, thus forcing the execution of separate analyses to compare the performance of different species in recognizing their targets. Here we introduce a new N, N-dimethylacrylamide-based polymeric coating, bearing simultaneously different functionalities (N-acryloyloxysuccinimide and azide groups) to allow an easy and straightforward method to co-immobilize proteins and oriented peptides on the same substrate. The bi-functional copolymer has been obtained by partial post polymerization modification of the functional groups of a common precursor. A NMR characterization of the copolymer was conducted to quantify the percentage of NAS that has been transformed into azido groups. The polymer was used to coat surfaces onto which both native antibodies and alkyne modified peptides were immobilized, to perform the phenotype characterization of extracellular vesicles (EVs). This strategy represents a convenient method to reduce the number of analysis, thus possible systematic or random errors, besides offering a drastic shortage in time, reagents and costs. (c) 2021 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据