期刊
ANALYTICA CHIMICA ACTA
卷 1195, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.aca.2022.339459
关键词
Photoelectrochemical immunoassay; Metal-organic frameworks; Ionic liquid; Gold nanoparticles; Alpha-fetoprotein
资金
- National Natural Science Foundation of China [21874157, 21675175, 22074160]
- Major Projects of Technical Innovation of Hubei Province [2017ACA172, 2020BCB032]
- Natural Science Foundation of Hubei Province [2018CFB617]
An ionic liquid with a sizeable heterocyclic structure was synthesized and used as a linker to coordinate with Eu3+ ions, forming metal-organic framework nanorods. By modifying with gold nanoparticles, the photoelectrochemical performance was enhanced, and a novel immunosensor for AFP detection was fabricated.
An ionic liquid with a sizeable heterocyclic structure was successfully synthesized and used as a linker to coordinate with Eu3+ ions to provide metal-organic framework (Eu-MOF) nanorods. Gold nanoparticlesmodified Eu-MOF nanocomposites (Eu-MOF@AuNPs) were synthesized by the in-situ reduction of chloroauric acid on the Eu-MOF nanorod surface. The outstanding conductivity and local surface plasmon resonance (LSPR) of Au-NPs and the distinct nature of MOFs worked together to enhance photoelectrochemical (PEC) performance by decreasing the recombination of photo-excited carriers and taking advantage of a broad absorption range for light harvesting. Under white light irradiation, the EuMOF@AuNPs nanocomposite had a better PEC performance than pure Eu-MOF. Therefore, a novel photoelectrochemical immunosensor was fabricated for alpha-fetoprotein (AFP) using Eu-MOF@AuNPs as the photoactive element. When the anti-AFP was attached to the Eu-MOF@AuNPs/GCE interface, the designed immunosensor exhibited a specific photocurrent response to AFP. The specific binding of AFP to anti-AFP can cause a significant steric hindrance, thus hinder the charge transfer and the separation of electron-hole pairs due to large molecular size and poor conductivity. As a result, the PEC immunosensing platform presented a decreasing trend on photocurrent intensity. Under the optimized conditions, the calibration plot showed a linearity with the logarithm of AFP concentration (0.002 e15.0 ng mL-1), and a detection limit of 0.16 pg mL-1 was obtained (S/N = 3). Moreover, this immunosensor presented a brilliant selectivity, high reproducibility, and outstanding stability, which possesses remarkable potential applications in determining AFP in serum samples. (c) 2022 Published by Elsevier B.V.
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