期刊
ANALYTICA CHIMICA ACTA
卷 1187, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.aca.2021.339143
关键词
Ruxolitinib; Molecularly imprinted polymer; Photopolymerization; Voltammetry; Drug analysis
资金
- Corman thanks the Scientific and Technological Research Council of Turkey (TUBITAK) under the BIDEB/2218-National Postdoctoral Research Fellowship Program for Turkish Citizens
- Council of Higher Education 100/2000 (YOK) under the special 100/2000 Programme
- Scientific and Technological Research Council of Turkey (TUBITAK) under the BIDEB/2211-A Ph.D. Scholarship Programme
A novel methodology was used to generate a porous molecularly imprinted material for highly selective and sensitive recognition of the Janus kinase inhibitor ruxolitinib (RUX). The porous MIP modified glassy carbon electrode linearly responded to RUX concentration up to 0.01 pM with a limit of detection of 0.00191 pM under optimized conditions. This method improves the accessibility of binding sites by generating porous material, resulting in highly selective and sensitive recognition of drugs in pharmaceutical dosage form and synthetic human serum samples.
A novel methodology has been applied to generate a porous molecularly imprinted material for highly selective and sensitive recognition of Janus kinase inhibitor ruxolitinib (RUX). The porous material-based nucleobase-derivative functional monomer was developed by a photopolymerization method. The thymine methacrylate (ThyM) as a functional monomer was synthesized and copolymerized with 2hydroxyethyl methacrylate (HEMA) in the presence of ethylene glycol dimethacrylate (EGDMA) onto the glassy carbon electrode [glassy carbon electrode/molecularly imprinted polymer@poly(2hydroxyethyl methacrylate-co-thymine methacrylate), (GCE/MIP@PHEMA-ThyM)] for the first time. The presence of ThyM results in the functional groups in imprinting binding sites, while the presence of poly(vinyl alcohol) (PVA) allows to generate porous materials for sensitive sensing. The characterization of GCE/MIP@PHEMA-ThyM was investigated by Fourier transform infrared (FT-IR) spectroscopy, scanning electron microscopy (SEM), and impedance spectroscopy technique. Then, the porous MIP modified glassy carbon electrode was optimized with effecting parameters including removal agent, removal time, and incubation time to get a better response for RUX. Under well-controlled optimum conditions, the GCE/MIP@PHEMA-ThyM linearly responded to the RUX concentration up to 0.01 pM at the limit of detection (LOD) of 0.00191 pM. The non-imprinted polymer (NIP) was also prepared to serve as a control in the same way but without the template. The proposed method improves the accessibility of binding sites by generating the porous material resulting in highly selective and sensitive recognition of drugs in the pharmaceutical dosage form and synthetic human serum samples. (c) 2021 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据