期刊
ANALYTICA CHIMICA ACTA
卷 1192, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.aca.2021.339380
关键词
Portable electrochemical sensor; Noradrenaline; In-vitro assay; Alzheimer diseases; PC12; Porous N-doped carbon materials
资金
- Japan Society for the Promotion of Science (JSPS) [P19067]
A portable electrochemical sensor was developed for monitoring noradrenaline (NA) secreted from living cells, showing high selectivity, sensitivity, and a wide linear range. The sensor design offers easy signaling, good stability, high biocompatibility, and compatibility with portable smartphones.
To date, the production and development of portable analytical devices for environmental and healthcare applications are rapidly growing. Herein, a portable electrochemical sensor for monitoring of noradrenaline (NA) secreted from living cells using mesoporous carbon-based materials was fabricated. The modification of the interdigitated electrode array (IDA) by nitrogen-doped mesoporous carbon spheres (N-doped MCS) and nitrogen-doped carbon hollow trunk-like structure (N-doped CHT) was used to fabricate the NA sensor. The N-doped CHT surface shows multiple holes distributed with micrometer sized open holes (1-2 mm) and nanometer-sized thin walls (similar to 98 nm). The N-doped CHT surface heterogeneity of wrinkled and twisted hollow trunk structures improve the diffusion pathway and the NA molecules loading. The N-doped CHT/IDA showed a highly selective assay for monitoring of NA with high sensitivity (1770 mA/mM x cm(2)), a low detection limit (0.005 mM), and a wide linear range (0.01-0.3 mM). The N-doped CHT/IDA monitored the NA secreted from PC12 cells under various concentrations of simulation agents (KCl). The designed N-doped CHT/IDA provides a portable NA-sensor assay with facile signaling, good stability, high biocompatibility, in-vitro assay compatibility, and good reproducibility. Therefore, the designed sensor can be used as a portable sensor for NA detection in live cells and can be matched with portable smartphones after further developments. (C) 2021 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据