4.3 Article

Combining UFLC-QTOF-MS analysis with biological evaluation of Centrosema coriaceum (Fabaceae) leaves

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ACAD BRASILEIRA DE CIENCIAS
DOI: 10.1590/0001-3765202220200491

关键词

anti-inflammatory; antioxidant; Candida glabrata; bioactivities; phenols

资金

  1. Fundacao de Amparo a Pesquisa do Estado de Minas Gerais [APQ-01059-14]
  2. Universidade Federal de Juiz de Fora (UFJF/Brazil)
  3. Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES, Brazil)

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This study determined the chemical profile of the ethanolic extract of C. coriaceum leaves using UFLC-QTOF-MS and evaluated its in vitro biological potential. The results showed that CCE exhibited antifungal, antioxidant, and anti-inflammatory activities.
Centrosema coriaceum Benth belongs to Fabaceae family and have few studies of biological activity and chemical composition. Thus, the aims of this work were to determine chemical profile of the ethanolic extract of C. coriaceum leaves (CCE) by UFLC-QTOF-MS and to evaluate its in vitro biological potential. CCE showed MIC value of 1000 mu g/mL against Candida glabrata (fungistatic effect) and high affinity in cell envelope by increasing cell permeability in nucleotide leakage, sorbitol and ergosterol assays. CCE showed antioxidant activity in all assays performed. For the anti-inflammatory and cytotoxicity activities, CCE, at all tested concentrations, significantly inhibited the production of nitric oxide and did not decrease J774A.1 cell viability below 70%. Finally, rutin, kaempferol-3O-rutinoside, caffeic acid, and sucrose were identified in CCE by UFLC-QTOF-MS. These results suggest, for the first time, that C. coriaceum has interesting antifungal, antioxidant, and anti-inflammatory activities.

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