期刊
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
卷 321, 期 6, 页码 H1005-H1013出版社
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/ajpheart.00477.2021
关键词
blebbistatin; cardiac physiology; excitation-contraction uncoupler; optical mapping
资金
- National Heart, Lung, and Blood Institute (NHLBI) [HL139472, HL111600, HL146169, HL147279]
- Children's Research Institute
- Children's National Heart Institute
- University of California Tobacco Related Disease Research Program Grant [T29IP0365C]
Optical mapping is a valuable imaging technique used in cardiovascular research to study electrophysiology and excitation-contraction coupling of cardiac tissues. The use of excitation-contraction uncoupling agents, such as blebbistatin, can help reduce signal distortion, but may have secondary effects on cardiac electrical activity and metabolic demand. It is essential to carefully consider the potential risks and best practices when using blebbistatin in optical mapping studies of cardiac tissue.
Optical mapping is an imaging technique that is extensively used in cardiovascular research, wherein parameter-sensitive fluorescent indicators are used to study the electrophysiology and excitation-contraction coupling of cardiac tissues. Despite many benefits of optical mapping, eliminating motion artifacts within the optical signals is a major challenge, as myocardial contraction interferes with the faithful acquisition of action potentials and intracellular calcium transients. As such, excitation-contraction uncoupling agents are frequently used to reduce signal distortion by suppressing contraction. When compared with other uncoupling agents, blebbistatin is the most frequently used, as it offers increased potency with minimal direct effects on cardiac electrophysiology. Nevertheless, blebbistatin may exert secondary effects on electrical activity, metabolism, and coronary flow, and the incorrect administration of blebbistatin to cardiac tissue can prove detrimental, resulting in erroneous interpretation of optical mapping results. In this Getting It Right perspective, we briefly review the literature regarding the use of blebbistatin in cardiac optical mapping experiments, highlight potential secondary effects of blebbistatin on cardiac electrical activity and metabolic demand, and conclude with the consensus of the authors on best practices for effectively using blebbistatin in optical mapping studies of cardiac tissue.
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