The cotton GhMYB4 gene enhances salt and drought tolerance in transgenic Arabidopsis

Salt and drought stresses are major environmental pressures in agriculture worldwide and affect crop productivity and quality. The previous reports of the R2R3-MYB transcription factor (TF) genes found that overexpression of these TF genes regulated the expression of key enzyme-encoding genes of flavonoid biosynthesis pathway, leading to the increased flavonoid accumulation. However, the roles of the cotton (Gossypium hirsutum L.) R2R3-MYB transcription factor gene GhMYB4 in regulating flavonoid accumulation and abiotic stress tolerance have not been investigated. In this study, the GhMYB4 gene was isolated from cotton. The observed subcellular localization in onion (Allium cepa L.) epidermal cells demonstrated that GhMYB4 was concentrated in the nucleus. A transactivation assay showed that this gene was transcriptionally active. The increased flavonoid accumulation and enhanced salt and drought tolerance of Arabidopsis overexpression plants were revealed. Under salt and drought stresses, proline and soluble sugar contents significantly increased, whereas H2O2 and malondialdehyde contents significantly decreased in transgenic plants. Further enzymatic analysis showed significant increases of superoxide dismutase, catalase, ascorbate peroxidase, and peroxidase activities in transgenic plants. Real-time quantitative polymerase chain reaction analysis showed that overexpression of GhMYB4 up-regulated the genes involved in flavonoid biosynthesis, proline biosynthesis, and reactive oxygen species scavenging in plants under salt and drought stresses. The overall results demonstrate that GhMYB4 has the potential to promote production of growth-benefiting flavonoids and improve tolerance to abiotic stresses in plants.

Automated summary

The isolated GhMYB4 gene from cotton was found to be localized in the nucleus, transcriptionally active, and overexpression of this gene led to increased flavonoid accumulation and improved tolerance to salt and drought stresses in plants. Furthermore, the overexpression of GhMYB4 up-regulated genes involved in flavonoid and proline biosynthesis, as well as reactive oxygen species scavenging, demonstrating its potential to enhance plant growth and stress tolerance.