期刊
ACTA BIOCHIMICA ET BIOPHYSICA SINICA
卷 54, 期 1, 页码 99-112出版社
SCIENCE PRESS
DOI: 10.3724/abbs.2021013
关键词
CENPM; lung adenocarcinoma; PI3K/AKT/mTOR signaling pathway
资金
- National Natural Science Foundation
- Regional Science Foundation Project [81960322]
- Medical Reserve Personnel Training Program of Yunnan Provincial Health Commission [H-2018097]
- Joint Program of Applied Basic Research of Yunnan Provincial Department of Science and Technology-Kunming Medical University [2018FE001(-247)]
- Medical Leading Talents Training Program of Yunnan Provincial Health Commission [L-2019028]
- Famous Doctor Special Project of the Ten Thousand People Plan of Yunnan Province [C20096]
- Kunming Medical University Master's Innovation Fund [2021S248]
This study found that CENPM is upregulated in LUAD and promotes its progression by facilitating cell cycle progression, cell proliferation, migration, and invasion, while inhibiting cell apoptosis. CENPM also affects the growth and metastasis of LUAD by promoting the phosphorylation of mTOR.
Centromere protein M (CENPM) is essential for chromosome separation during mitosis. However, its roles in lung adenocarcinoma (LUAD) progression and metastasis remain unknown. In this study, we aimed to explore the effects of CENPM on LUAD progression as well as the underlying mechanisms. We analyzed the expression of CENPM and its correlation with clinicopathological characteristics using GEO LUAD chip datasets and TCGA dataset. We further investigated the impact of CENPM on LUAD in vitro and in vivo. In silico analysis and qRT-PCR revealed that CENPM is upregulated in LUAD compared with that in normal lung tissues. Via gain/loss-of-function assays, we further found that CENPM promotes the LUAD cell cycle, cell proliferation, migration and invasion, and inhibits cell apoptosis in vitro. The in vivo study showed that loss of CENPM inhibits the growth of A549 xenografts. Furthermore, we found that CENPM can promote the phosphorylation of mTOR rather than directly affect the mTOR content. Inhibition of mTOR activity abrogates the promoting effects of CENPM on cell cycle progression, cell proliferation, migration and invasion. Taken together, these results show that CENPM plays an important role in the growth and metastasis of LUAD and may be a promising therapeutic target in LUAD.
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