期刊
ACS NANO
卷 16, 期 1, 页码 1589-1599出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsnano.1c10255
关键词
biological nanofluidic device; single-protein sensitivity; bacteriorhodopsin; a single SiNx nanopore; proton-pumping activity
类别
资金
- National Natural Science Foundation of China [21675146, 21475125]
- National Key Research and Development Program of China [2016YFA0201300]
- Instrument Developing Project of the Chinese Academy of Sciences [YZ201666]
This study reports a method for probing the bioactivity of functional proteins using a biological nanofluidic device, achieving detection at the single-molecule level. The method enables higher charge density, suggesting potential applications in biosensing and energy conversion.
Nanofluidics is an emerging hot field that explores the unusual behaviors of ions/molecules transporting through nanoscale channels, which possesses a broad application prospect. However, in situ probing bioactivity of functional proteins on a single-molecule level by a nanofluidic device has not been reported, and it is still a big challenge in the field. Herein, we reported a biological nanofluidic device with a single-protein sensitivity, based on natural proton-pumping protein, bacteriorhodopsin (bR), and a single SiNx nanopore. Nanofluidic single-molecule probing of bR proton-pumping activity and its light response were achieved under applied voltage of 0 V, by biologically self-powered steady-state ionic current nanopore sensing. Green-light irradiation of the device led to the monitoring of a steady-state proton current of similar to 3.51 pA/per bR trimer, corresponding to charge density of 815 mu C/cm(2) generated by each bR monomer, which far exceeded the previously reported value of 1.4 mu C/cm(2). This finding and method would promote the development of artificial biological and hybrid nanofluidic devices in biosensing and energy conversion applications.
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