期刊
ACS NANO
卷 16, 期 2, 页码 2164-2175出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsnano.1c08103
关键词
silicification; mammalian cells; protein accessibility; enzyme bioactivity; long-term preservation
类别
资金
- NIH [FP0003261, CA226537]
- National Natural Science Foundation of China [21972047]
- Guangdong Provincial Pearl River Talents Program [2019QN01Y314]
- Program for Guangdong Introducing Innovative and Entrepreneurial Teams [2019ZT08Y318]
Freezing cells in amorphous silica is an effective method for long-term preservation of mammalian cell proteomic structure and function at room temperature, avoiding issues related to cryopreservation.
Preservation of evolved biological structure and function in robust engineering materials is of interest for storage of biological samples before diagnosis and development of vaccines, sensors, and enzymatic reactors and has the potential to avoid cryopreservation and its associated cold-chain issues. Here, we demonstrate that freezing cells in amorphous silica is a powerful technique for long-term preservation of whole mammalian cell proteomic structure and function at room temperature. Biomimetic silicification employs the crowded protein microenvironment of mammalian cells as a catalytic framework to proximally transform monomeric silicic acid into silicates forming a nanoscopic silica shell over all biomolecular interfaces. Silicification followed by dehydration preserves and passivates proteomic information within a nanoscale thin silica coating that exhibits size selective permeability (<3.6 nm), preventing protein leaching and protease degradation of cellular contents, while providing access of small molecular constituents for cellular enzymatic reaction. Exposure of dehydrated silicified cells to mild etchant or prolonged hydrolysis removes the silica, completely rerevealing biomolecular components and restoring their accessibility and functionality.
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