期刊
ACS NANO
卷 15, 期 11, 页码 17453-17462出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsnano.1c03236
关键词
optodiagnostic; low-cost diagnostic; cotton swabs; gold nanoparticles; colorimetric sensor; SARS-CoV-2; COVID-19
类别
资金
- AIChE Foundation
- Penn Mental Health AIDS Research Center of the University of Pennsylvania
- National Institute of General Medical Sciences of the National Institutes of Health [R35GM138201]
- Defense Threat Reduction Agency (DTRA) [HDTRA11810041, HDTRA1-21-1-0014]
- Perelman School of Medicine at the University of Pennsylvania
- CAPES [88887.479793/2020-00]
- FAPESP [2018/08782-1]
- CNPq [438828/2018-6, 401256/2020-0]
- Institute for Diabetes, Obesity, and Metabolism
The COVID-19 pandemic has widened the health equity gap in society, disproportionately affecting disadvantaged populations. A low-cost rapid testing device called COLOR can detect SARS-CoV-2 within 5 minutes, providing a solution for high-frequency testing in low-resource communities.
The COVID-19 pandemic has exacerbated our society's tremendous health equity gap. Disadvantaged populations have been disproportionally affected by COVID-19, lacking access to affordable testing, a known effective tool for preventing viral spread, hospitalizations, and deaths. Here, we describe COVID-19 Low-cost Optodiagnostic for Rapid testing (COLOR), a colorimetric biosensor fabricated on cotton swabs using gold nanoparticles modified with human angiotensin-converting enzyme 2 (ACE2), which costs 15 phi to produce and detects SARS-CoV-2 within 5 min. COLOR detected very low viral particle loads (limit of detection: 0.154 pg mL(-1) of SARS-CoV-2 spike protein), and its color intensity correlated with the cycle threshold (Ct) values obtained using reverse transcription polymerase chain reaction (RT-PCR). The performance of COLOR was assessed using 100 nasopharyngeal/oropharyngeal (NP/OP) clinical samples, yielding sensitivity, specificity, and accuracy values of 96%, 84%, and 90%, respectively. In summary, each COLOR test can be manufactured for 15 phi and presents rapid minute-time scale detection of SARS-CoV-2, thus providing a solution to enable high-frequency testing, particularly in low-resource communities.
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