期刊
ACS APPLIED MATERIALS & INTERFACES
卷 13, 期 42, 页码 49642-49657出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.1c11787
关键词
periodontitis; periodontal regeneration; inflammation; infection; electrospinning; oral bacteria
资金
- National Institutes of Health (NIH), the National Institute of Dental and Craniofacial Research [K08DE023552, R01DE026578]
- Osteology Foundation
- OsteoScience Foundation (Peter Geistlich Research Award)
- International Association for Dental Research (IADR-GSK Innovation in Oral Care Award)
- American Academy of Implant Dentistry Foundation
This study developed personalized antibiotic-laden scaffolds for periodontitis treatment using electrospinning techniques. Evaluation through chemo-morphological analyses, cytocompatibility assessment, and antimicrobial validation showed promising results for promoting new bone formation, reducing bone loss, and lowering inflammatory response. The fabrication of defect-specific antibiotic-laden scaffolds holds potential for personalized medication and regenerative properties.
Periodontitis compromises the integrity and function of tooth-supporting structures. Although therapeutic approaches have been offered, predictable regeneration of periodontal tissues remains intangible, particularly in anatomically complex defects. In this work, personalized and defect-specific antibiotic-laden polymeric scaffolds containing metronidazole (MET), tetracycline (TCH), or their combination (MET/TCH) were created via electrospinning. An initial screening of the synthesized fibers comprising chemo-morphological analyses, cytocompatibility assessment, and antimicrobial validation against periodontopathogens was accomplished to determine the cell-friendly and anti-infective nature of the scaffolds. According to the cytocompatibility and antimicrobial data, the 1:3 MET/TCH formulation was used to obtain three-dimensional defect-specific scaffolds to treat periodontally compromised three-wall osseous defects in rats. Inflammatory cell response and new bone formation were assessed by histology. Micro-computerized tomography was performed to assess bone loss in the furcation area at 2 and 6 weeks post implantation. Chemo-morphological and cell compatibility analyses confirmed the synthesis of cytocompatible antibioticladen fibers with antimicrobial action. Importantly, the 1:3 MET/TCH defect-specific scaffolds led to increased new bone formation, lower bone loss, and reduced inflammatory response when compared to antibiotic-free scaffolds. Altogether, our results suggest that the fabrication of defect-specific antibiotic-laden scaffolds holds great potential toward the development of personalized (i.e., patientspecific medication) scaffolds to ablate infection while affording regenerative properties.
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