期刊
ACS APPLIED MATERIALS & INTERFACES
卷 14, 期 4, 页码 5615-5623出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.1c22809
关键词
bifunctional Zr-MOF; visual fluorescence response; electrochemical ppb-level sensitivity; dual-channel assay; Ochratoxin A; food safety monitoring
资金
- National Natural Science Foundation of China [31772073, 60901079]
- Natural Science Foundation of Jiangsu Province [BE2019359]
- Jiangsu Specially-Appointed Professor Project [20TPJS002]
- Postdoctoral Science Foundation of China [2021 M691319]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
This study proposes a fluorescence and electrochemical dual-channel biosensor based on bifunctional Zr-MOF for detecting OTA. The sensor utilizes the photoluminescence properties and enormous electroactive ligands of Zr-MOF to load OTA-specific aptamers, simplifying the fabrication process and improving sensing reliability. The dual-channel signal change enables intuitive fluorescence strategy and sensitive electrochemical analysis.
A simple fluorescence and electrochemical dual- channel biosensor based on bifunctional Zr(IV)-based metal-organic framework (Zr-MOF) was proposed to detect Ochratoxin A (OTA). The bifunctional Zr-MOF, with photoluminescence properties and enormous electroactive ligands, was exploited to load OTA-specific aptamers for designing signal probes, greatly simplifying the probe-fabrication process and improving sensing reliability. Upon specific recognition of aptamer toward OTA, the anchored probe was released from the sensing interface into the reaction solution. In this circumstance, the increased amount of the signal probe in reaction solution led to an enhanced fluorescence response, while the decreased amount of the signal probe on the sensing interface resulted in a diminished electrochemical response. According to the dual-channel signal change with increasing OTA concentration, the visual fluorescence strategy was established for intuitive OTA detection, and meanwhile, sensitive electrochemical assay with a detection limit of 0.024 pg/mL was also achieved with the help of one-step electrodeposition as a sensing platform. Moreover, the proposed dual-channel assay has been successfully applied to determine OTA levels in corn samples with rapid response, superior accuracy, and high antiinterference capability, providing a promising method for food safety monitoring.
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